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Multicenter Study
. 2024 Sep 1;137(3):473-493.
doi: 10.1152/japplphysiol.00102.2024. Epub 2024 Apr 18.

Molecular Transducers of Physical Activity Consortium (MoTrPAC): human studies design and protocol

MoTrPAC Study Group; John M Jakicic  1 , Wendy M Kohrt  2 , Joseph A Houmard  3 , Michael E Miller  4 , Shlomit Radom-Aizik  5 , Blake B Rasmussen  6 , Eric Ravussin  7 , Monica Serra  6 , Cynthia L Stowe  8 , Scott Trappe  9 , Hiba Abouassi  10 , Joshua N Adkins  11 , D Lee Alekel  12 , Euan Ashley  13 , Marcas M Bamman  14 , Bryan C Bergman  2 , Daniel H Bessesen  2 , Nicholas T Broskey  3 , Thomas W Buford  14 , Charles F Burant  15 , Haiying Chen  4 , Jeffrey W Christle  13 , Clary B Clish  16 , Paul M Coen  17 , David Collier  3 , Katherine A Collins  10 , Daniel M Cooper  5 , Tiffany Cortes  6 , Gary R Cutter  18 , Gabriel Dubis  3 , Facundo M Fernández  19 , Jonathon Firnhaber  3 , Daniel E Forman  20 , David A Gaul  19 , Nicole Gay  21 , Robert E Gerszten  16   22 , Bret H Goodpaster  17 , Marina A Gritsenko  11 , Fadia Haddad  5 , Kim M Huffman  10 , Olga Ilkayeva  23 , Catherine M Jankowski  2 , Christopher Jin  21 , Neil M Johannsen  7 , Johanna Johnson  10 , Leslie Kelly  10 , Erin Kershaw  20 , William E Kraus  4 , Maren Laughlin  24 , Bridget Lester  9 , Malene E Lindholm  13 , Adam Lowe  7 , Ching-Ju Lu  25 , Joan McGowan  12 , Edward L Melanson  2 , Stephen Montgomery  26 , Samuel G Moore  19 , Kerrie L Moreau  2 , Michael Muehlbauer  23 , Nicolas Musi  27 , Venugopalan D Nair  28 , Christopher B Newgard  23 , Anne B Newman  20 , Barbara Nicklas  4 , Bradley C Nindl  20 , Kelly Ormond  13   29 , Paul D Piehowski  11 , Wei-Jun Qian  11 , Tuomo Rankinen  7 , W Jack Rejeski  30 , Jeremy Robbins  22 , Renee J Rogers  1 , Jessica L Rooney  31 , Scott Rushing  4 , James A Sanford  11 , Irene E Schauer  2 , Robert S Schwartz  2 , Stuart C Sealfon  28 , Cris Slentz  10 , Ruben Sloan  3 , Kevin S Smith  26 , Michael Snyder  21 , Jessica Spahn  32 , Lauren M Sparks  17 , Maja Stefanovic-Racic  20 , Charles J Tanner  3 , Anna Thalacker-Mercer  14 , Russell Tracy  31 , Todd A Trappe  9 , Elena Volpi  6 , Martin J Walsh  28 , Matthew T Wheeler  13 , Leslie Willis  10
Affiliations
Multicenter Study

Molecular Transducers of Physical Activity Consortium (MoTrPAC): human studies design and protocol

MoTrPAC Study Group et al. J Appl Physiol (1985). .

Abstract

Physical activity, including structured exercise, is associated with favorable health-related chronic disease outcomes. Although there is evidence of various molecular pathways that affect these responses, a comprehensive molecular map of these molecular responses to exercise has not been developed. The Molecular Transducers of Physical Activity Consortium (MoTrPAC) is a multicenter study designed to isolate the effects of structured exercise training on the molecular mechanisms underlying the health benefits of exercise and physical activity. MoTrPAC contains both a preclinical and human component. The details of the human studies component of MoTrPAC that include the design and methods are presented here. The human studies contain both an adult and pediatric component. In the adult component, sedentary participants are randomized to 12 wk of Control, Endurance Exercise Training, or Resistance Exercise Training with outcomes measures completed before and following the 12 wk. The adult component also includes recruitment of highly active endurance-trained or resistance-trained participants who only complete measures once. A similar design is used for the pediatric component; however, only endurance exercise is examined. Phenotyping measures include weight, body composition, vital signs, cardiorespiratory fitness, muscular strength, physical activity and diet, and other questionnaires. Participants also complete an acute rest period (adults only) or exercise session (adults, pediatrics) with collection of biospecimens (blood only for pediatrics) to allow for examination of the molecular responses. The design and methods of MoTrPAC may inform other studies. Moreover, MoTrPAC will provide a repository of data that can be used broadly across the scientific community.NEW & NOTEWORTHY The Molecular Transducers of Physical Activity Consortium (MoTrPAC) will be the first large trial to isolate the effects of structured exercise training on the molecular mechanisms underlying the health benefits of exercise and physical activity. By generating a compendium of the molecular responses to exercise, MoTrPAC will lay the foundation for a new era of biomedical research on Precision Exercise Medicine. Presented here is the design, protocols, and procedures for the MoTrPAC human studies.

Keywords: adipose tissue; biospecimens; molecular transducers; physical activity; skeletal muscle.

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Conflict of interest statement

Dr. J. M. Jakicic is a member of the Scientific Advisory Board for Wondr Health, Inc. None of the other authors has any conflicts of interest, financial or otherwise, to disclose.

Figures

None
Graphical abstract
Figure 1.
Figure 1.
MoTrPAC organizational scheme. MoTrPAC, Molecular Transducers of Physical Activity Consortium.
Figure 2.
Figure 2.
Design of adult and pediatric studies within MoTrPAC. MoTrPAC, Molecular Transducers of Physical Activity Consortium.
Figure 3.
Figure 3.
Clinic flow and procedures for adult participants.
Figure 4.
Figure 4.
Clinic flow and procedures for pediatric participants.
Figure 5.
Figure 5.
Timing of biospecimen collection. CON, control intervention; EE, endurance exercise; PA, physical activity.
Figure 6.
Figure 6.
MoTrPAC data flow and quality control. CRF, case report form; DMAQC, Data Management, Analysis, and Quality Control; MoTrPAC, Molecular Transducers of Physical Activity Consortium; QC, quality control.
See this image and copyright information in PMC

References

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