doi: 10.1016/0378-1119(85)90255-0.
Complete nucleotide sequence of the Azotobacter vinelandii nitrogenase structural gene cluster
- PMID: 3863780
- DOI: 10.1016/0378-1119(85)90255-0
Item in Clipboard
Complete nucleotide sequence of the Azotobacter vinelandii nitrogenase structural gene cluster
Gene.
1985.
Abstract
DNA fragments coding for the structural genes for Azotobacter vinelandii nitrogenase have been isolated and sequenced. These genes, nifH, nifD and nifK, code for the iron (Fe) protein and the alpha and beta subunits of the molybdenum-iron (MoFe) protein, respectively. They are arranged in the order: promoter:nifH:nifD:nifK. There are 129 nucleotides separating nifH and nifD and 101 nucleotides separating nifD and nifK. The amino acid (aa) sequences deduced from the nucleotide sequences are discussed in relation to the prosthetic group-binding regions of the nifHDK-encoded polypeptides.
Similar articles
-
Products of the iron-molybdenum cofactor-specific biosynthetic genes, nifE and nifN, are structurally homologous to the products of the nitrogenase molybdenum-iron protein genes, nifD and nifK.J Bacteriol. 1987 Apr;169(4):1547-53. doi: 10.1128/jb.169.4.1547-1553.1987. J Bacteriol. 1987. PMID: 3470285 Free PMC article.
-
Distinct structural features of the alpha and beta subunits of nitrogenase molybdenum-iron protein of Clostridium pasteurianum: an analysis of amino acid sequences.Biochemistry. 1988 Apr 19;27(8):2800-10. doi: 10.1021/bi00408a021. Biochemistry. 1988. PMID: 2840948
-
The nifH gene encoding the Fe protein component of the molybdenum nitrogenase from Azotobacter chroococcum.Gene. 1993 Jan 15;123(1):145-6. doi: 10.1016/0378-1119(93)90555-h. Gene. 1993. PMID: 8423000
-
Iron-molybdenum cofactor biosynthesis in Azotobacter vinelandii requires the iron protein of nitrogenase.J Biol Chem. 1987 Oct 15;262(29):14327-32. J Biol Chem. 1987. PMID: 3477546
-
Nitrogenase structure and function: a biochemical-genetic perspective.Annu Rev Microbiol. 1995;49:335-66. doi: 10.1146/annurev.mi.49.100195.002003. Annu Rev Microbiol. 1995. PMID: 8561464 Review.
Cited by
-
Nucleotide sequence of the frxC, petB and trnL genes in the chloroplast genome of Chlamydomonas reinhardtii.Plant Mol Biol. 1992 Mar;18(5):985-8. doi: 10.1007/BF00019214. Plant Mol Biol. 1992. PMID: 1581576 No abstract available.
-
Site-directed mutagenesis of the Klebsiella pneumoniae nitrogenase. Effects of modifying conserved cysteine residues in the alpha- and beta-subunits.Biochem J. 1989 Nov 15;264(1):257-64. doi: 10.1042/bj2640257. Biochem J. 1989. PMID: 2557830 Free PMC article.
-
The Azorhizobium caulinodans nifA gene: identification of upstream-activating sequences including a new element, the 'anaerobox'.Nucleic Acids Res. 1988 Oct 25;16(20):9839-53. doi: 10.1093/nar/16.20.9839. Nucleic Acids Res. 1988. PMID: 3186446 Free PMC article.
-
Characterization of the gene for the Fe-protein of the vanadium dependent alternative nitrogenase of Azotobacter vinelandii and construction of a Tn5 mutant.Mol Gen Genet. 1988 Sep;214(1):121-7. doi: 10.1007/BF00340189. Mol Gen Genet. 1988. PMID: 3226421
-
Tn5-induced mutants of Azotobacter vinelandii affected in nitrogen fixation under Mo-deficient and Mo-sufficient conditions.J Bacteriol. 1986 Nov;168(2):673-82. doi: 10.1128/jb.168.2.673-682.1986. J Bacteriol. 1986. PMID: 3023285 Free PMC article.
Publication types
MeSH terms
Substances
Associated data
- Actions
LinkOut - more resources
Full Text Sources
