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. 2024 Apr 20;147(1):74.
doi: 10.1007/s00401-024-02731-z.

Nanopore sequencing from formalin-fixed paraffin-embedded specimens for copy-number profiling and methylation-based CNS tumor classification

Ann-Kristin Afflerbach  1   2   3 , Anne Albers  4 , Anton Appelt  1   2 , Leonille Schweizer  5   6   7 , Werner Paulus  4 , Michael Bockmayr  1   2   8 , Ulrich Schüller #  1   2   9 , Christian Thomas #  10
Affiliations

Nanopore sequencing from formalin-fixed paraffin-embedded specimens for copy-number profiling and methylation-based CNS tumor classification

Ann-Kristin Afflerbach et al. Acta Neuropathol. .
No abstract available

Keywords: CNS tumors; Formalin-fixed paraffin-embedded; Nanopore sequencing.

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Conflict of interest statement

The author declare no conflict of interest.

Figures

Fig. 1
Fig. 1
Sample overview and results of FFPE DNA nanopore sequencing analysis. a Sample overview of cohort with clinical characteristics as well as analysis results. b CNV heatmap of analyzed samples, with losses in blue and gains in red. Samples show expected chromosomal alterations, especially glioblastomas (GBM) with the characteristic +7/−10 signature and co-deletion of Chr 1p and 19q in all oligodendrogliomas (OL). Samples #28 and #32 did not produce enough data for sufficient CNV representation. c Comparison of EPIC CNV plots (top) and Nanopore CNV plots (bottom) for samples #8 and #9, showing high concordance between the two methods
See this image and copyright information in PMC

References

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