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. 2024 Apr 20;20(1):150.
doi: 10.1186/s12917-024-04002-1.

Milk miRNA expression in buffaloes as a potential biomarker for mastitis

Abhishek B Jadhav  1 , Shailesh D Ingole  2 , Simin V Bharucha  1 , Korsapati L Yoshitha  1 , Rajiv V Gaikwad  3 , Rajesh R Pharande  4 , Shambhudeo D Kharde  1
Affiliations

Milk miRNA expression in buffaloes as a potential biomarker for mastitis

Abhishek B Jadhav et al. BMC Vet Res. .

Abstract

Background: Buffaloes have the highest potential for production due to a promising gene pool that is being enhanced and upgraded. Mastitis is a significant health impediment that greatly diminishes milk yield and quality, affecting rural farmers' livelihoods. The traditional gold standard used for diagnosing mastitis or subclinical mastitis is CMT, but it has the drawback of false positive or negative results. Subclinical mastitis, if not treated promptly, can lead to mammary tumors. To address the gap in early diagnosis of subclinical mastitis in CMT-negative milk of buffaloes, we performed a retrospective analysis and evaluated the milk miRNA expression profiles as potential biomarkers.

Results: Thirty buffalo milk samples based on clinical signs and CMT were divided into normal, subclinical, and clinical mastitis. SCC evaluation showed significant differences between the groups. The data analysis demonstrated that the elevation of miR-146a and miR-383 differed substantially between normal, subclinical, and clinical mastitis milk of buffaloes with 100% sensitivity and specificity. The relationship of SCC with miR-146a and miR-383 in normal/healthy and subclinical mastitis was positively correlated.

Conclusion: The overexpression of miR-146a and miR-383 is associated with inflammation. It can be a valuable prognostic and most sensitive biomarker for early mastitis detection in buffaloes with SCC below 2 lakhs and CMT-ve, enhancing the accuracy of subclinical mastitis diagnosis.

Keywords: Buffaloes; Mastitis; Milk; miRNA.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
Box and Whisker plots for miR-146a expression in milk by qRT-PCR between normal, subclinical, and clinical mastitis buffaloes. Horizontal lines in the box are median values, and X are mean values
Fig. 2
Fig. 2
Box and Whisker plots for miR-383 expression in milk by qRT-PCR between normal, subclinical, and clinical mastitis buffaloes. Horizontal lines in the box are median values, and X are mean values
Fig. 3
Fig. 3
ROC curve for miR-146a in the clinical mastitis group
Fig. 4
Fig. 4
ROC curve of miR-146a in the subclinical mastitis group
Fig. 5
Fig. 5
ROC curve of miR-383 in the clinical mastitis group
Fig. 6
Fig. 6
ROC curve of miR-383 in the subclinical mastitis group
See this image and copyright information in PMC

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