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. 2023 Jun;7(6):237.
doi: 10.3390/jcs7060237. Epub 2023 Jun 6.

PGS/Gelatin Nanocomposite Electrospun Wound Dressing

Mahyar Naseri  1 , Aysan Hedayatnazari  2 , Lobat Tayebi  1
Affiliations

PGS/Gelatin Nanocomposite Electrospun Wound Dressing

Mahyar Naseri et al. J Compos Sci. 2023 Jun.

Abstract

Infectious diabetic wounds can result in severe injuries or even death. Biocompatible wound dressings offer one of the best ways to treat these wounds, but creating a dressing with a suitable hydrophilicity and biodegradation rate can be challenging. To address this issue, we used the electrospinning method to create a wound dressing composed of poly(glycerol sebacate) (PGS) and gelatin (Gel). We dissolved the PGS and Gel in acetic acid (75 v/v%) and added EDC/NHS solution as a crosslinking agent. Our measurements revealed that the scaffolds' fiber diameter ranged from 180.2 to 370.6 nm, and all the scaffolds had porosity percentages above 70%, making them suitable for wound healing applications. Additionally, we observed a significant decrease (p < 0.05) in the contact angle from 110.8° ± 4.3° for PGS to 54.9° ± 2.1° for PGS/Gel scaffolds, indicating an improvement in hydrophilicity of the blend scaffold. Furthermore, our cell viability evaluations demonstrated a significant increase (p < 0.05) in cultured cell growth and proliferation on the scaffolds during the culture time. Our findings suggest that the PGS/Gel scaffold has potential for wound healing applications.

Keywords: diabetic wound; electrospun wound dressing; gelatin; poly(glycerol sebacate); regenerative medicine; skin tissue engineering.

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Conflict of interest statement

Conflicts of Interest: The authors declare no conflict of interest.

Figures

Figure 1.
Figure 1.
The schematic of the synthesis process of PGS prepolymer.
Figure 2.
Figure 2.
The SEM micrographs of (a,b) PGS and (c,d) PGS/Gel.
Figure 3.
Figure 3.
The FTIR spectra of (a) PGS and (b) PGS/Gel.
Figure 4.
Figure 4.
The water contact angle measurements of the samples.
Figure 5.
Figure 5.
The weight loss measurements of the samples immersed in the PBS solution.
Figure 6.
Figure 6.
The MTT results of the samples (* indicate p value < 0.05).
Figure 7.
Figure 7.
The SEM micrographs of the PGS/Gel scaffold on the 5th day of cell culture at (a) 250×, (b) 500×, (c) 2000×, and (d) 4000× magnifications.
See this image and copyright information in PMC

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