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. 2022 Mar 21;9(1):29.
doi: 10.1186/s40643-022-00521-7.

De novo biosynthesis of τ-cadinol in engineered Escherichia coli

Yue Sun  1   2 , Shaoting Wu  2 , Xiao Fu  2 , Chongde Lai  3 , Daoyi Guo  4
Affiliations

De novo biosynthesis of τ-cadinol in engineered Escherichia coli

Yue Sun et al. Bioresour Bioprocess. .

Abstract

τ-Cadinol is a sesquiterpene that is widely used in perfume, fine chemicals and medicines industry. In this study, we established a biosynthetic pathway for the first time in engineered Escherichia coli for production of τ-cadinol from simple carbon sources. Subsequently, we further improved the τ-cadinol production to 35.9 ± 4.3 mg/L by optimizing biosynthetic pathway and overproduction of rate-limiting enzyme IdI. Finally, the titer was increased to 133.5 ± 11.2 mg/L with a two-phase organic overlay-culture medium system. This study shows an efficient method for the biosynthesis of τ-cadinol in E. coli with the heterologous hybrid MVA pathway.

Keywords: Escherichia coli; MVA; Metabolic engineering; τ-Cadinol.

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
Engineered pathway for the production of τ-cadinol from glucose
Fig. 2
Fig. 2
The time course of cell OD600 and τ-cadinol concentration of engineered E. coli strains in shake flasks. A SY01 strain; B SY02 strain; C SY03 strain; D SY03 strain with overlay-solvent
Fig. 3
Fig. 3
The time course of glucose concentration of engineered E. coli strains in shake flasks
See this image and copyright information in PMC

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