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. 2024 Jan 18;11(1):12.
doi: 10.1186/s40643-023-00726-4.

Non-targeted discovery of high-value bio-products in Nicotiana glauca L: a potential renewable plant feedstock

Natalia Carreno-Quintero  1   2 , Takayuki Tohge  3 , Rebecca Van Acker  4   5 , Lauren S McKee  6   7 , Qi Zhou  6   7 , Antje Bolze  3 , Xiaohui Xing  6 , Merve Özparpucu  8   9 , Markus Rüggeberg  8   9 , Thomas Piofczyk  10 , Yaw Koram  11 , Vincent Bulone  6   12 , Wout Boerjan  4   5 , Alisdair R Fernie  13 , Paul D Fraser  14
Affiliations

Non-targeted discovery of high-value bio-products in Nicotiana glauca L: a potential renewable plant feedstock

Natalia Carreno-Quintero et al. Bioresour Bioprocess. .

Abstract

The evaluation of plant-based feedstocks is an important aspect of biorefining. Nicotiana glauca is a solanaceous, non-food crop that produces large amounts of biomass and is well adapted to grow in suboptimal conditions. In the present article, compatible sequential solvent extractions were applied to N. glauca leaves to enable the generation of enriched extracts containing higher metabolite content comparing to direct leaf extracts. Typically, between 60 to 100 metabolite components were identified within the fractions. The occurrence of plant fatty acids, fatty acid alcohols, alkanes, sterols and terpenoids was detected by gas liquid chromatography-mass spectrometry (GC-MS) and metabolite identification was confirmed by comparison of physico-chemical properties displayed by available authentic standards. Collectively, co-products such waxes, oils, fermentable sugars, and terpenoids were all identified and quantified. The enriched fractions of N. glauca revealed a high level of readily extractable hydrocarbons, oils and high value co-products. In addition, the saccharification yield and cell wall composition analyses in the stems revealed the potential of the residue material as a promising lignocellulosic substrate for the production of fermentable sugars. In conclusion a multifractional cascade for valuable compounds/commodities has been development, that uses N. glauca biomass. These data have enabled the evaluation of N. glauca material as a potential feedstock for biorefining.

Keywords: Nicotiana glauca; Bioproducts; Biorefinary; Metabolite profiling.

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Conflict of interest statement

Thomas Piofczyk and Yaw Koram work for private sector companies, however, have no relevant competing interests to declare.

Figures

Fig. 1
Fig. 1
Gas chromatography profile of a non-polar metabolites of non-polar extracts1 and 2 and b polar metabolites derived from mature leaves of N. glauca after large scale extraction. ME: Methyl Esther. For non-polar metabolites numbers are: 1. C16:0 ME, 2. ALA 3TMS, 3. C16:0 ME, 4. C18:2 ME, 5. phytol, 6. C18:2, 7. ALA 3TMS, 8. C18:0, 9. tetracosanol-1-ol. 10. hentriacontane, 11. β-sitosterol. For Polar metabolites, numbers are: 1. fructose, 2. glucose, 3. myo-inositol, 4. sucrose, 5. glycerol, 6. malate, 7. succinate, 8. glycerate, 9. pyroglutamate, 10. galactose, 11. unknown sugar
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