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. 2025 May;35(5):2837-2844.
doi: 10.1007/s10895-024-03642-8. Epub 2024 Apr 22.

Development of a Coumarin-derived Fluorescent Probe for Detection of HOCl and its Application in Cells and Zebrafish

Xin Chen  1 , Jie Chen  2 , Shanshan Wang  2 , Shihua Yu  1 , Zhigang Liu  3 , Xiaodan Zeng  4
Affiliations

Development of a Coumarin-derived Fluorescent Probe for Detection of HOCl and its Application in Cells and Zebrafish

Xin Chen et al. J Fluoresc. 2025 May.

Abstract

We have prepared a simple, universal and efficient coumarin-derived fluorescent probe (XDS1) to detecting HOCl. The experimental findings revealed that the introduction of HOCl produced an obvious quenching effect on the probe with high selectivity and sensitivity. The calculated limit of detection (LOD) was as low as 0.02 μM. Furthermore, an impressive response time of less than 10 s was observed when XDS1 detecting HOCl. Importantly, the probe XDS1 exhibited negligible cytotoxicity, thereby facilitating its application for imaging HOCl within biological environment. The probe XDS1 had been successfully used for specific detection in cells.

Keywords: Bioimaging; Coumarin; Fluorescent probe; HOCl.

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Conflict of interest statement

Declarations. Ethics Approval and Consent to Participate: Not required. Consent for Publication: Not Applicable. Competing Interests: The authors declare no competing interest.

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