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. 2024:2790:417-426.
doi: 10.1007/978-1-0716-3790-6_22.

Purification of Rubisco from Leaves

Joana Amaral  1 , Ana K M Lobo  1 , Elizabete Carmo-Silva  1 , Douglas J Orr  2
Affiliations

Purification of Rubisco from Leaves

Joana Amaral et al. Methods Mol Biol. 2024.

Abstract

Rubisco fixes CO2 through the carboxylation of ribulose 1,5-bisphosphate (RuBP) during photosynthesis, enabling the synthesis of organic compounds. The natural diversity of Rubisco properties represents an opportunity to improve its performance and there is considerable research effort focusing on better understanding the properties and regulation of the enzyme. This chapter describes a method for large-scale purification of Rubisco from leaves. After the extraction of Rubisco from plant leaves, the enzyme is separated from other proteins by fractional precipitation with polyethylene glycol followed by ion-exchange chromatography. This method enables the isolation of Rubisco in large quantities for a wide range of biochemical applications.

Keywords: CO2 fixation; Calvin-Benson-Bassham cycle; Chromatography; Enzyme catalysis; Protein extraction; Ribulose-1,5-bisphosphate carboxylase/oxygenase.

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References

    1. Tcherkez G (2013) Modelling the reaction mechanism of ribulose-1,5-bisphosphate carboxylase/oxygenase and consequences for kinetic parameters. Plant Cell Environ 36:1586–1596 - DOI - PubMed
    1. Tcherkez GG, Farquhar GD, Andrews TJ (2006) Despite slow catalysis and confused substrate specificity, all ribulose bisphosphate carboxylases may be nearly perfectly optimized. Proc Natl Acad Sci USA 103:7246–7251 - DOI - PubMed - PMC
    1. Raines CA (2003) The Calvin cycle revisited. Photosynth Res 75:1–10 - DOI - PubMed
    1. Andersson I (2008) Catalysis and regulation in Rubisco. J Exp Bot 59:1555–1568 - DOI - PubMed
    1. Carmo-Silva E, Scales JC, Madgwick PJ, Parry MAJ (2015) Optimizing Rubisco and its regulation for greater resource use efficiency. Plant Cell Environ 38:1817–1832 - DOI - PubMed

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