Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 Jun;242(6):2652-2668.
doi: 10.1111/nph.19770. Epub 2024 Apr 22.

MicroRNA164e suppresses NAC100 transcription factor-mediated synthesis of seed storage proteins in chickpea

Anirban Chakraborty  1 , Baljinder Singh  1 , Vimal Pandey  1 , Swarup K Parida  1 , Sabhyata Bhatia  1
Affiliations
Free article

MicroRNA164e suppresses NAC100 transcription factor-mediated synthesis of seed storage proteins in chickpea

Anirban Chakraborty et al. New Phytol. 2024 Jun.
Free article

Abstract

Development of protein-enriched chickpea varieties necessitates an understanding of specific genes and key regulatory circuits that govern the synthesis of seed storage proteins (SSPs). Here, we demonstrated the novel involvement of Ca-miR164e-CaNAC100 in regulating SSP synthesis in chickpea. Ca-miRNA164e was significantly decreased during seed maturation, especially in high-protein accessions. The miRNA was found to directly target the transactivation conferring C-terminal region of a nuclear-localized transcription factor, CaNAC100 as revealed using RNA ligase-mediated-rapid amplification of cDNA ends and target mimic assays. The functional role of CaNAC100 was demonstrated through seed-specific overexpression (NACOE) resulting in significantly augmented seed protein content (SPC) consequential to increased SSP transcription. Further, NACOE lines displayed conspicuously enhanced seed weight but reduced numbers and yield. Conversely, a downregulation of CaNAC100 and SSP transcripts was evident in seed-specific overexpression lines of Ca-miR164e that culminated in significantly lowered SPC. CaNAC100 was additionally demonstrated to transactivate the SSP-encoding genes by directly binding to their promoters as demonstrated using electrophoretic mobility shift and dual-luciferase reporter assays. Taken together, our study for the first time established a distinct role of CaNAC100 in positively influencing SSP synthesis and its critical regulation by CamiR164e, thereby serving as an understanding that can be utilized for developing SPC-rich chickpea varieties.

Keywords: NAC100; chickpea; microRNA164; seed protein content; seed storage proteins; transactivation.

PubMed Disclaimer

References

    1. Bai X, Huang Y, Hu Y, Liu H, Zhang B, Smaczniak C, Hu G, Han Z, Xing Y. 2017. Duplication of an upstream silencer of FZP increases grain yield in rice. Nature Plants 3: 885–893.
    1. Basu U, Parida SK. 2023. The developmental dynamics in cool season legumes with focus on chickpea. Plant Molecular Biology 111: 473–491.
    1. Basu U, Upadhyaya HD, Srivastava R, Daware A, Malik N, Sharma A, Bajaj D, Narnoliya L, Thakro V, Kujur A et al. 2019. Abc transporter-mediated transport of glutathione conjugates enhances seed yield and quality in chickpea. Plant Physiology 180: 253–275.
    1. Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein‐dye binding. Analytical Biochemistry 72: 248–254.
    1. Brousse C, Liu Q, Beauclair L, Deremetz A, Axtell MJ, Bouché N. 2014. A non‐canonical plant microRNA target site. Nucleic Acids Research 42: 5270–5279.

MeSH terms

LinkOut - more resources