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. 2021 Dec 17;8(1):130.
doi: 10.1186/s40643-021-00476-1.

Vinasse: from a residue to a high added value biopolymer

Daiana V Trapé  1   2 , Olivia V López  1   3 , Marcelo A Villar  4   5
Affiliations

Vinasse: from a residue to a high added value biopolymer

Daiana V Trapé et al. Bioresour Bioprocess. .

Abstract

This work aimed to study the feasibility of using vinasse for polyhydroxybutyrate (PHB) production by Bacillus megaterium. To optimize the culture medium, a Box-Behnken design was employed considering carbon (C), nitrogen (N), and phosphorus (Ph) concentrations as independent variables and PHB productivity as the response variable. The productivity decreased when C or N were increased, probably due to the presence of phenolic compounds and the limitation of N for the production of PHB by Bacillus sp. bacteria. An additional experimental design to optimize the C/N ratio and growing conditions (fermentation time and temperature) was carried out. Fermentation time had a statistically significant effect on PHB productivity reaching 10.6 mg/L h. On the other hand, the variability in physicochemical properties of vinasse samples led to significant differences in PHB productivity. Lower productivity values were obtained when vinasse had higher values of DBO. Therefore, biopolymers production from vinasse is a feasible alternative to valorize this bioethanol by-product.

Keywords: Bacillus megaterium; Culture medium optimization; Microbial fermentation; Polyhydroxybutyrate (PHB); Vinasse.

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
Flowchart of bioethanol production process
Fig. 2
Fig. 2
Response surface representing the effect of carbon and nitrogen concentration in culture medium on PHB productivity
Fig. 3
Fig. 3
Response surface representing the effect of a C/N ratio and time, b temperature and time, and c C/N ratio and temperature on PHB productivity
Fig. 4
Fig. 4
SEM micrograph and FTIR spectrum of PHA synthesized by B. megaterium using vinasse as carbon source
See this image and copyright information in PMC

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