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. 1985 Nov;82(22):7580-4.
doi: 10.1073/pnas.82.22.7580.

Cloning and expression of the human erythropoietin gene

F K Lin, S Suggs, C H Lin, J K Browne, R Smalling, J C Egrie, K K Chen, G M Fox, F Martin, Z Stabinsky, et al.

Cloning and expression of the human erythropoietin gene

F K Lin et al. Proc Natl Acad Sci U S A. 1985 Nov.

Abstract

The human erythropoietin gene has been isolated from a genomic phage library by using mixed 20-mer and 17-mer oligonucleotide probes. The entire coding region of the gene is contained in a 5.4-kilobase HindIII-BamHI fragment. The gene contains four intervening sequences (1562 base pairs) and five exons (582 base pairs). It encodes a 27-amino acid signal peptide and a 166-amino acid mature protein with a calculated Mr of 18,399. The erythropoietin gene, when introduced into Chinese hamster ovary cells, produces erythropoietin that is biologically active in vitro and in vivo.

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References

    1. Mol Cell Biol. 1982 Feb;2(2):161-70 - PubMed
    1. Proc Natl Acad Sci U S A. 1983 Feb;80(3):802-6 - PubMed
    1. Biochem Soc Symp. 1974;(40):17-26 - PubMed
    1. Br J Haematol. 1977 Sep;37(1):85-91 - PubMed
    1. Br Med J. 1965 Oct 30;2(5469):1036-8 - PubMed

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