Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1985 Nov;82(22):7580-4.
doi: 10.1073/pnas.82.22.7580.

Cloning and expression of the human erythropoietin gene

F K Lin, S Suggs, C H Lin, J K Browne, R Smalling, J C Egrie, K K Chen, G M Fox, F Martin, Z Stabinsky, et al.

Cloning and expression of the human erythropoietin gene

F K Lin et al. Proc Natl Acad Sci U S A. 1985 Nov.

Abstract

The human erythropoietin gene has been isolated from a genomic phage library by using mixed 20-mer and 17-mer oligonucleotide probes. The entire coding region of the gene is contained in a 5.4-kilobase HindIII-BamHI fragment. The gene contains four intervening sequences (1562 base pairs) and five exons (582 base pairs). It encodes a 27-amino acid signal peptide and a 166-amino acid mature protein with a calculated Mr of 18,399. The erythropoietin gene, when introduced into Chinese hamster ovary cells, produces erythropoietin that is biologically active in vitro and in vivo.

PubMed Disclaimer

References

    1. Mol Cell Biol. 1982 Feb;2(2):161-70 - PubMed
    1. Proc Natl Acad Sci U S A. 1983 Feb;80(3):802-6 - PubMed
    1. Biochem Soc Symp. 1974;(40):17-26 - PubMed
    1. Br J Haematol. 1977 Sep;37(1):85-91 - PubMed
    1. Br Med J. 1965 Oct 30;2(5469):1036-8 - PubMed

Publication types

Associated data