Complete genome sequence of the Streptomyces bacteriophage Amabiko

Abstract

Amabiko is a lytic subcluster BE2 bacteriophage that infects Streptomyces scabiei-a bacterium causing common scab in potatoes. Its 131,414 bp genome has a GC content of 49.5% and contains 245 putative protein-coding genes, 45 tRNAs, and one tmRNA. Amabiko is closely related to Streptomyces bacteriophage MindFlayer (gene content similarity: 86.5%).

Keywords: bacteriophage assembly.

Fig 1

Characteristics of the Streptomyces bacteriophage Amabiko. (a) Amabiko forms ~2 mm clear, symmetrical, circular plaques. (b) Negative-stain (1% uranyl acetate) transmission electron microscopy image of Amabiko. Amabiko exhibits a siphoviral morphology, with a head length and width of 77 nm and an uncontracted tail length of 333 nm (n = 1; scale bar is located in the corner of the image). (c) Amabiko's complete genome sequence, containing 245 putative genes. The ruler indicates the length of the genome in kilobase pairs. Boxes represent individual genes transcribed in the forward and reverse direction (above and below the ruler, respectively), with purple boxes indicating genes that could be assigned a putative function and orange boxes indicating genes of unknown function. tRNAs and the tmRNA are shown as red boxes.