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. 1985 Dec;82(23):7904-8.
doi: 10.1073/pnas.82.23.7904.

Exposure of actin thiols by the removal of tightly held calcium ions

K Konno, M F Morales

Exposure of actin thiols by the removal of tightly held calcium ions

K Konno et al. Proc Natl Acad Sci U S A. 1985 Dec.

Abstract

The removal of bound metal ions from G-actin uncovered two thiols, Cys-10 and Cys-257. The uncovering of these thiols requires a free calcium concentration lower than 10 nM. Therefore, participation of one or both thiols in Ca2+ binding is suggested. Actin labeled with N-(5-fluoresceinyl)maleimide in the absence of calcium moves as a doublet in NaDodSO4/PAGE. It is suggested that two conformers are induced by metal removal and labeling.

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