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. 2024 Jun 15;209(12):1501-1504.
doi: 10.1164/rccm.202310-1831LE.

Human Airway and Alveolar Organoids from BAL Fluid

Monica Yun Liu  1   2   3 , Belinda Chen  1 , Mehdi Borji  4   5 , Carolina Garcia de Alba Rivas  1 , Antonella F M Dost  1   6 , Aaron L Moye  1 , Nusrath Movval Abdulla  2 , Margherita Paschini  1 , Stuart D Rollins  7   8 , Ruobing Wang  7   8 , Lynn M Schnapp  3 , Hassan A Khalil  9 , Catherine J Wu  4   5   8 , Nirmal S Sharma  2   10 , Carla F Kim  1   7   11   12
Affiliations

Human Airway and Alveolar Organoids from BAL Fluid

Monica Yun Liu et al. Am J Respir Crit Care Med. .
No abstract available

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Figures

Figure 1.
Figure 1.
Airway organoids from BAL fluid. (A) Representative phase contrast image of an airway organoid 7–10 days after initial plating (passage 0). After expansion, immunofluorescence on whole organoids shows predominant expression of basal cell markers TP63 (green) and KRT5 (magenta). Scale bars, 100 μm. (B) Representative flow cytometry at passage 2 shows selective outgrowth of EpCAM+ NGFR+ airway basal cells. The NGFR+ population formed a majority across all samples analyzed. (C) Uniform Manifold Approximation and Projection (UMAP) of airway organoids derived from two different human BAL samples and normal lung tissue, and distribution of cell populations in each airway organoid culture. Inset shows subclustering of the small ionocyte/tuft/neuroendocrine population. (D) Dot plot of canonical cell markers.
Figure 2.
Figure 2.
Alveolar organoids from BAL fluid. (A) Representative phase contrast image of an alveolar organoid 7–10 days after initial plating (passage 0). After expansion, immunofluorescence on sectioned organoids shows expression of the alveolar type 2 (AT2) cell markers surfactant protein C (SPC; green) and HT2-280 (magenta) on the expected apical surface. Scale bars, 100 μm. (B) Representative flow cytometry at passage 2 shows selective outgrowth of EpCAM+ HT2-280+ AT2 cells. The HT2-280+ population was variable across all samples analyzed and could be sorted. (C) Uniform Manifold Approximation and Projection (UMAP) of alveolar organoids derived from two different human BAL samples and normal lung tissue, and distribution of cell populations in each alveolar organoid culture. BAL 1 is an unsorted alveolar organoid culture, whereas BAL 2 organoids were grown from HT2-280+ sorted cells; tissue-derived organoids were not sorted. (D) Dot plot of canonical cell markers.
See this image and copyright information in PMC

References

    1. Liberti DC, Morrisey EE. Organoid models: assessing lung cell fate decisions and disease responses. Trends Mol Med . 2021;27:1159–1174. - PubMed
    1. Sachs N, Papaspyropoulos A, Zomer-van Ommen DD, Heo I, Böttinger L, Klay D, et al. Long-term expanding human airway organoids for disease modeling. EMBO J . 2019;38:1–20. - PMC - PubMed
    1. Liu MY, Garcia De Alba Rivas C, Movval Abdulla N, Sharma NS, Kim CF. Establishing airway lung organoids from human bronchoalveolar lavage fluid [abstract] Am J Respir Crit Care Med . 2023;207:A4690.
    1. Maestre-Batlle D, Pena OM, Hirota JA, Gunawan E, Rider CF, Sutherland D, et al. Novel flow cytometry approach to identify bronchial epithelial cells from healthy human airways. Sci Rep . 2017;7:42214. - PMC - PubMed
    1. Konishi S, Tata A, Tata PR. Defined conditions for long-term expansion of murine and human alveolar epithelial stem cells in three-dimensional cultures. STAR Protoc . 2022;3:101447. - PMC - PubMed

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