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. 2024 Apr 23;191(5):283.
doi: 10.1007/s00604-024-06336-x.

Rapid detection of melamine by DNA Walker mediated SERS sensing technique based on signal amplification function

Yujing Ma  1   2 , Han Cui  1   2 , Ruipeng Chen  2 , Rui Zhang  1   2 , Jiaqi Lin  2 , Shuyue Ren  3 , Jun Liang  4 , Zhixian Gao  5
Affiliations

Rapid detection of melamine by DNA Walker mediated SERS sensing technique based on signal amplification function

Yujing Ma et al. Mikrochim Acta. .

Abstract

A new method is proposed for detecting typical melamine dopants in food using surface-enhanced Raman scattering (SERS) biosensing technology. Melamine specific aptamer was used as the identification probe, and gold magnets (AuNPs@MNPs) and small gold nanoparticles (AuNPs@MBA) were used as the basis for Raman detection. The Raman signal of the detection system can directly detect melamine quantitatively. Under optimized conditions, the detection of melamine was carried out in the low concentration range of 0.001-500 mg/kg, the enhancement factor (EF) was 2.3 × 107, and the detection limit was 0.001 mg/kg. The method is sensitive and rapid, and can be used for the rapid detection of melamine in the field environment.

Keywords: Aptamer; DNA walker; Melamine; Surface enhanced Raman sensing technology.

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