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. 2024 Apr 9:11:1284097.
doi: 10.3389/fvets.2024.1284097. eCollection 2024.

Evaluation of an ultrasound-guided freeze-core biopsy system for canine and feline brain tumors

Brian S Adams  1 , Dominic J Marino  1 , Catherine A Loughin  1 , Leonard J Marino  1 , Teresa Southard  2 , Martin L Lesser  3 , Meredith Akerman  3 , Patrick Roynard  1   4
Affiliations

Evaluation of an ultrasound-guided freeze-core biopsy system for canine and feline brain tumors

Brian S Adams et al. Front Vet Sci. .

Abstract

Objective: To determine if a single brain biopsy utilizing a freeze-core needle harvest system Cassi II under ultrasound guidance provides a diagnostic sample; to evaluate the technique's efficacy in procuring diagnostic samples in comparison with "open" surgical biopsies; and to describe intraoperative complications associated with the technique.

Study design: Experimental clinical study.

Animals: Seventeen dogs and four cats with magnetic resonance imaging (MRI) diagnoses of readily surgically accessible intracranial masses.

Methods: Immediately prior to surgical biopsy (SB), freeze-core biopsy (FCB) sample was obtained from each patient under ultrasound guidance.

Results: Histopathology results from single FCB samples were found to be in 100% agreement with the SB samples. Freezing artifact was minimal and did not interfere with histopathologic interpretation. There were no intraoperative complications specifically attributable to the use of the FCB system.

Conclusion: Based on the results of this small experimental study, the FCB system is expected to safely yield diagnostic quality intracranial masses biopsy specimens.

Clinical significance: This system has the potential of obtaining diagnostic biopsies of more deeply seated brain lesions (i.e., intra-axial tumors considered inaccessible or with large risks/difficulties by standard surgical means) which would provide a definitive diagnosis to guide appropriate therapy.

Keywords: MRI; brain tumor; freeze-core biopsy; ultrasound-guided; veterinary.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

Figure 1
Figure 1
The Sanarus Cassi II freeze-core biopsy system utilizes a CO2 cartridge (a) to provide cooling for the quick “stick freeze” and a combined securing needle and a rotating cutting cannula (b). The hand piece contains the operational control buttons (c).
Figure 2
Figure 2
The securing needle (a) stabilizes the specimen using contact freezing (b) while the rotating cutting cannula advances and retracts revealing the biopsy specimen (c). Typical surgical set-up and positioning for biopsy (d).
Figure 3
Figure 3
Meningioma removed from the parietal lobe of a 9-year-old DSH. Transverse T1W post-contrast (left) and T2W (right) MR images (A) showing the large mass (red arrow). In both the surgical biopsy (B) and the Cassi biopsy (C) the mass was diagnosed as a meningioma. The Cassi biopsy samples have shrunken nuclei with darker staining chromatin and mild loss of nuclear and cytoplasmic detail compared with surgical biopsy samples.
See this image and copyright information in PMC

References

    1. Dewey CW. Encephalopathies: disorders of the brain. In:Dewey CW, and Da Costa, RC, editors. Practical Guide to Canine and Feline Neurology. Ames, IA: Wiley Blackwell; (2016). p. 141–236.
    1. LeCouteur R. Brain biopsy in dogs & cats. In: World Small Animal Veterinary Association World Congress Proceedings. Available online at: https://www.vin.com/doc/?id=5124410 (accessed August 6, 2023).
    1. Wolff CA, Holmes SP, Young BD, Chen AV, Kent M, Platt SR, et al. . Magnetic resonance imaging for the differentiation of neoplastic, inflammatory, and cerebrovascular brain disease in dogs. J Vet Intern Med. (2012) 26:589–97. 10.1111/j.1939-1676.2012.00899.x - DOI - PubMed
    1. Cervera V, Mai W, Vite C, Johnson V, Dayrell-Hart B, Seiler G. Comparative magnetic resonance imaging findings between gliomas and presumed cerebrovascular accidents in dogs. Vet Radiol Ultras. (2010) 52:33–40. 10.1111/j.1740-8261.2010.01749.x - DOI - PubMed
    1. Di Terlizzi R, Platt SR. The function, composition and analysis of cerebrospinal fluid in companion animals: part II—analysis. Vet J. (2009) 180:15–32. 10.1016/j.tvjl.2007.11.024 - DOI - PubMed