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. 2024 Apr 17:2024:2501086.
doi: 10.1155/2024/2501086. eCollection 2024.

The Association between Histidine-Rich Glycoprotein rs10770 Genotype and Recurrent Miscarriage in Iranian Women

Mahbobeh Latifimehr  1 , Leila Nazari  2 , Ali Asghar Rastegari  1 , Zahra Zamani  3 , Pezhman Fard-Esfahani  3
Affiliations

The Association between Histidine-Rich Glycoprotein rs10770 Genotype and Recurrent Miscarriage in Iranian Women

Mahbobeh Latifimehr et al. Biomed Res Int. .

Abstract

Purpose: Recurrent miscarriage (RM) is a significant reproductive concern affecting numerous women globally. Genetic factors are believed to play a crucial role in RM, making the histidine-rich glycoprotein (HRG) gene, a topic of interest due to its potential involvement in angiogenesis. This study is aimed at investigating the association between the HRG rs10770 genotype and RM.

Method: Blood samples were collected from a total of 200 women at the beginning of the study. Subsequently, a comparative analysis was conducted between the blood samples of 100 women with a history of RM (case group) and the blood samples of another 100 healthy women (control group). HRG rs10770 genotyping was performed through polymerase chain reaction restriction-fragment length polymorphism (PCR-RFLP), followed by statistical analysis to evaluate the relationship between HRG rs10770 genotype and RM.

Results: The results indicated a significant statistical difference between the C/C genotype (OR = 3.32, CI: 1.22-9.04, p = 0.01) and the C/T genotype (OR = 1.24, CI: 0.67-2.30, p = 0.47) in both the case and control groups. Additionally, a significant correlation was observed in the C allelic frequency among RM participants compared to the control group (OR = 1.65, CI: 1.06-2.58, p = 0.02).

Conclusion: The study highlights the importance of HRG rs10770 in understanding RM, shedding light on its implications for reproductive health. Furthermore, it became evident that women carrying the homozygous C/C genotype exhibited increased susceptibility to the risk of RM.

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Conflict of interest statement

The authors declare that they have no conflicts of interest.

Figures

Figure 1
Figure 1
Agarose gel electrophoresis after PCR. L: 100 bp DNA ladder; 1, 2, 3, 4, 5, 6, and 7 show the 376 bp fragment of HRG gene.
Figure 2
Figure 2
Agarose gel electrophoresis after RFLP. Lanes: L, 100 bp DNA ladder; 1, 2, and 3, homozygous T/T; 4 and 7, heterozygous C/T; 5 and 6, homozygous C/C.
See this image and copyright information in PMC

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