Investigating cryopreserved PBMC functionality in an antigen-induced model of sarcoidosis granuloma formation

Sarah G Seman¹, Sabahattin Bicer², Mark W Julian³, Jonah R Mitchell⁴, Patrick J Kramer⁵, Elliott D Crouser⁶, Landon W Locke⁷

Affiliations

¹ Biomedical Engineering Department, The Ohio State University College of Engineering, The Ohio State University, Columbus, OH, USA. Electronic address: seman.40@osu.edu.
² Biomedical Engineering Department, The Ohio State University College of Engineering, The Ohio State University, Columbus, OH, USA. Electronic address: bicer.1@osu.edu.
³ Division of Pulmonary, Critical Care and Sleep Medicine, The Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center, Columbus, OH, USA. Electronic address: mark.julian@osumc.edu.
⁴ Biomedical Engineering Department, The Ohio State University College of Engineering, The Ohio State University, Columbus, OH, USA. Electronic address: mitchell.1714@osu.edu.
⁵ Division of Pulmonary, Critical Care and Sleep Medicine, The Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center, Columbus, OH, USA. Electronic address: patrick.kramer@osumc.edu.
⁶ Division of Pulmonary, Critical Care and Sleep Medicine, The Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center, Columbus, OH, USA. Electronic address: elliott.crouser@osumc.edu.
⁷ Biomedical Engineering Department, The Ohio State University College of Engineering, The Ohio State University, Columbus, OH, USA. Electronic address: locke.51@osu.edu.

PMID: 38663096
DOI: 10.1016/j.bbrc.2024.149993

Free article

Investigating cryopreserved PBMC functionality in an antigen-induced model of sarcoidosis granuloma formation

Sarah G Seman et al. Biochem Biophys Res Commun. 2024.

Free article

. 2024 Jun 25:714:149993.

doi: 10.1016/j.bbrc.2024.149993. Epub 2024 Apr 23.

Authors

Sarah G Seman¹, Sabahattin Bicer², Mark W Julian³, Jonah R Mitchell⁴, Patrick J Kramer⁵, Elliott D Crouser⁶, Landon W Locke⁷

Affiliations

¹ Biomedical Engineering Department, The Ohio State University College of Engineering, The Ohio State University, Columbus, OH, USA. Electronic address: seman.40@osu.edu.
² Biomedical Engineering Department, The Ohio State University College of Engineering, The Ohio State University, Columbus, OH, USA. Electronic address: bicer.1@osu.edu.
³ Division of Pulmonary, Critical Care and Sleep Medicine, The Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center, Columbus, OH, USA. Electronic address: mark.julian@osumc.edu.
⁴ Biomedical Engineering Department, The Ohio State University College of Engineering, The Ohio State University, Columbus, OH, USA. Electronic address: mitchell.1714@osu.edu.
⁵ Division of Pulmonary, Critical Care and Sleep Medicine, The Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center, Columbus, OH, USA. Electronic address: patrick.kramer@osumc.edu.
⁶ Division of Pulmonary, Critical Care and Sleep Medicine, The Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center, Columbus, OH, USA. Electronic address: elliott.crouser@osumc.edu.
⁷ Biomedical Engineering Department, The Ohio State University College of Engineering, The Ohio State University, Columbus, OH, USA. Electronic address: locke.51@osu.edu.

PMID: 38663096
DOI: 10.1016/j.bbrc.2024.149993

Abstract

Sarcoidosis, a systemic inflammatory disease, poses challenges in understanding its etiology and variable clinical courses. Despite ongoing uncertainty about causative agents and genetic predisposition, granuloma formation remains its hallmark feature. To address this, we developed a validated in vitro human granuloma model using patient-derived peripheral blood mononuclear cells (PBMCs), offering a dynamic platform for studying early granuloma formation and sarcoidosis pathogenesis. However, a current limitation of this model is its dependence on freshly isolated PBMCs obtained from whole blood. While cryopreservation is a common method for long-term sample preservation, the biological effects of freezing and thawing PBMCs on granuloma formation remain unclear. This study aimed to assess the viability and functionality of cryopreserved sarcoidosis PBMCs within the granuloma model, revealing similar granulomatous responses to fresh cells and highlighting the potential of cryopreserved PBMCs as a valuable tool for studying sarcoidosis and related diseases.

Keywords: Cryopreservation; Disease modeling; Granuloma formation; Peripheral blood mononuclear cells; Sarcoidosis.

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Conflict of interest statement

Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

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Investigating cryopreserved PBMC functionality in an antigen-induced model of sarcoidosis granuloma formation

Affiliations

Investigating cryopreserved PBMC functionality in an antigen-induced model of sarcoidosis granuloma formation

Authors

Affiliations

Abstract

Conflict of interest statement

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MeSH terms

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LinkOut - more resources

Full Text Sources

Medical