Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 Apr;12(4):e2438.
doi: 10.1002/mgg3.2438.

Mother's smoking habits affects IL10 methylation but not asthma in Ecuadorian children

Cristina Ochoa-Avilés  1   2 , Angélica Ochoa-Avilés  1 , Roque Rivas-Párraga  1   3 , Samuel Escandón  1 , Talita Dos Santos-Jesus  2 , Milca de J Silva  2 , Valderiene Leão  2 , Marco Salinas  3 , Yosselin Vicuña  4 , Lucy Baldeón  4 , María José Molina-Cando  1   5 , Diana Morillo  1   5 , Marcos Machuca  6 , Claudia Rodas  6 , Camila Figueiredo  2 , Vivian Alejandra Neira  1   6
Affiliations

Mother's smoking habits affects IL10 methylation but not asthma in Ecuadorian children

Cristina Ochoa-Avilés et al. Mol Genet Genomic Med. 2024 Apr.

Abstract

There is no evidence evaluating the IL10 epigenetic upregulation among mestizo children in a high-altitude Andean city in Latin America.

Objective: To identify polymorphisms and methylation profiles in the IL10 gene associated with asthma in children aged 5 to 11.

Methods: A case-control study was conducted with asthmatic and non-asthmatic children aged 5 to 11 years in Cuenca-Ecuador. Data on allergic diseases and risk factors were collected through a questionnaire for parents. Atopy was measured by skin prick test (SPT) to relevant aeroallergens. Three IL10 single nucleotide polymorphisms were evaluated in all participants, and methylation analysis was performed in 54 participants. Association between risk factors, allergic diseases and genetic factors were estimated using multivariate logistic regression.

Results: The results of polymorphisms showed no differences between cases and controls when comparing the SNPs rs3024495, rs3024496, rs1800896 allelic and genotypic frequencies. In the methylation analysis, no differences in the IL10 methylation profile were found between cases and controls; however, the multivariate analysis showed an association between the mother's smoking habits and the IL10 methylation profile.

Conclusion: Smoking habit could be essential as an environmental exposure factor in regulating gene expression in children with asthma.

Keywords: IL10; asthma; children; methylation; polymorphisms.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

Figures

FIGURE 1
FIGURE 1
LD plot of the IL10 SNPs analyzed in asthmatic and control patients. The top horizontal bar illustrates thelocation of SNPs on a physical scale. The color of the squares illustrates the strength of pairwise r 2 values on a scale where black indicates perfect LD (r 2 = 1), shades of gray indicate imperfect LD (0 < r 2 < 1) and white indicates perfect equilibrium (r 2 = 0). The r 2 LD value is also indicated within each square.
FIGURE 2
FIGURE 2
Distribution of cases and controls with respect to methylation percentage.
See this image and copyright information in PMC

References

    1. Bousquet, J. , Heinzerling, L. , Bachert, C. , Papadopoulos, N. G. , Bousquet, P. J. , Burney, P. G. , Canonica, G. W. , Carlsen, K. H. , Cox, L. , Haahtela, T. , Lodrup Carlsen, K. C. , Price, D. , Samolinski, B. , Simons, F. E. , Wickman, M. , Annesi‐Maesano, I. , Baena‐Cagnani, C. E. , Bergmann, K. C. , Bindslev‐Jensen, C. , … Allergic Rhinitis and its Impact on Asthma . (2012). Practical guide to skin prick tests in allergy to aeroallergens. Allergy, 67(1), 18–24. 10.1111/j.1398-9995.2011.02728.x - DOI - PubMed
    1. Chilmonczyk, B. A. , Salmun, L. M. , Megathlin, K. N. , Neveux, L. M. , Palomaki, G. E. , Knight, G. J. , Pulkkinen, A. J. , & Haddow, J. E. (1993). Association between exposure to environmental tobacco smoke and exacerbations of asthma in children. The New England Journal of Medicine, 328(23), 1665–1669. 10.1056/NEJM199306103282303 - DOI - PubMed
    1. Cooper, P. J. , Rodrigues, L. C. , Cruz, A. A. , & Barreto, M. L. (2009). Asthma in Latin America: A public heath challenge and research opportunity. Allergy, 64(1), 5–17. 10.1111/j.1398-9995.2008.01902.x - DOI - PubMed
    1. Delgado, O. (2013). El plan de desarrollo y ordenamiento territorial del cantón cuenca, Azuay. https://dspace.ups.edu.ec/bitstream/123456789/11170/1/El%20plan%20de%20d...
    1. Figueiredo, C. A. , Barreto, M. L. , Alcantara‐Neves, N. M. , Cooper, P. J. , Rodrigues, L. C. , Cruz, A. A. , Pontes‐de‐Carvalho, L. C. , Vergara, C. , Rafaels, N. , Gao, L. , Foster, C. , Campbell, M. , Mathias, R. A. , & Barnes, K. C. (2012). Co‐associations between IL10 genetic variants, IL 10 production and helminth infection in a tropical population of Brazil with high prevalence of asthma. The Journal of Allergy and Clinical Immunology, 129(2), AB62. https://www.jacionline.org/article/S0091‐6749(11)02689‐3/abstract

Publication types