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. 1985;201(2):168-73.
doi: 10.1007/BF00425655.

Methylation of 16S ribosomal RNA and resistance to the aminoglycoside antibiotics gentamicin and kanamycin determined by DNA from the gentamicin-producer, Micromonospora purpurea

J Thompson, P A Skeggs, E Cundliffe

Methylation of 16S ribosomal RNA and resistance to the aminoglycoside antibiotics gentamicin and kanamycin determined by DNA from the gentamicin-producer, Micromonospora purpurea

J Thompson et al. Mol Gen Genet. 1985.

Abstract

When DNA fragments from Micromonospora purpurea (the producer of gentamicin) were cloned in Streptomyces lividans, a gentamicin-resistant strain was obtained in which the ribosomes were highly resistant both to gentamicin and to kanamycin. Reconstitution analysis revealed that such resistance resulted from some property of their 16S RNA. Extracts from the clone contained methylase activity which acted on 16S RNA within E. coli 30S ribosomal subunits and rendered them resistant to gentamicin and kanamycin.

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References

    1. J Gen Microbiol. 1985 Mar;131(3):451-7 - PubMed
    1. J Bacteriol. 1982 Aug;151(2):668-77 - PubMed
    1. J Antibiot (Tokyo). 1981 Sep;34(9):1175-82 - PubMed
    1. Biochem Biophys Res Commun. 1981 Jun 16;100(3):1396-401 - PubMed
    1. Br Med Bull. 1984 Jan;40(1):61-7 - PubMed

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