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. 2024 Apr 17;13(8):1120.
doi: 10.3390/plants13081120.

Response Surface Methodology for Optimization of Ultrasound-Assisted Antioxidants Extraction from Blackberry, Chokeberry and Raspberry Pomaces

Iga Piasecka  1 , Rita Brzezińska  1 , Stanisław Kalisz  2 , Artur Wiktor  3 , Agata Górska  1
Affiliations

Response Surface Methodology for Optimization of Ultrasound-Assisted Antioxidants Extraction from Blackberry, Chokeberry and Raspberry Pomaces

Iga Piasecka et al. Plants (Basel). .

Abstract

An investigation of the ultrasound-assisted extraction (UAE) of polyphenol-rich aqueous extracts from blackberry, chokeberry and raspberry pomaces was carried out. The aim of the study was to choose optimal conditions for UAE in order to obtain extracts rich in phenolic compounds. The optimization was carried out based on response surface methodology. The variable conditions were amplitude of ultrasound wave and extraction time, whereas responses were total polyphenol content and antioxidant capacity. Based on the ANOVA analysis, mathematical models were fitted and verified. The most effective conditions of amplitude and time were 98% and 5.00 min, 78% and 10.32 min and 90% and 11.56 min for blackberry pomace, chokeberry pomace and raspberry pomace, respectively. The actual results obtained in optimized conditions were comparable to the results predicted by the models. Additionally, the anthocyanin content in extracts was determined in the high-performance liquid chromatography assay. It was proven that response surface methodology could be a useful tool in the optimization of UAE processes for obtaining polyphenol-rich extracts from berry fruit pomaces.

Keywords: by-products; polyphenols; pomace; response surface methodology; ultrasound-assisted extraction.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Three-dimensional and contour graphs showing total polyphenol content dependence from extraction time and ultrasound amplitude.
Figure 2
Figure 2
Three-dimensional and contour graphs showing antioxidant capacity dependence from extraction time and ultrasound amplitude.
Figure 3
Figure 3
Graphs illustrating correlation between TPC and antioxidant capacity (ABTS) of pomace extracts. Dashed line marks 95% confidence interval.
See this image and copyright information in PMC

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