Comparative Study
doi: 10.1089/dna.1985.4.469.
Detection of chloramphenicol acetyl transferase activity in transfected cells: a rapid and sensitive HPLC-based method
- PMID: 3867497
- DOI: 10.1089/dna.1985.4.469
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Comparative Study
Detection of chloramphenicol acetyl transferase activity in transfected cells: a rapid and sensitive HPLC-based method
DNA.
1985 Dec.
Abstract
An assay based on high-performance liquid chromatography (HPLC) has been developed for determining the expression of the acetyl coenzyme A: chloramphenicol 3-O-acetyltransferase gene in transfected cells. The assay is based on the separation of extracted, nonradioactive chloramphenicol and its acetylated derivatives by reverse-phase HPLC, using UV absorbance for detection. In addition to eliminating the need for costly [14C]chloramphenicol, this assay is as sensitive and much faster than the commonly used method involving thin-layer chromatography.
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