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Comparative Study
. 1985 Dec;4(6):469-75.
doi: 10.1089/dna.1985.4.469.

Detection of chloramphenicol acetyl transferase activity in transfected cells: a rapid and sensitive HPLC-based method

S L Young, A E Jackson, D Puett, M H Melner
Comparative Study

Detection of chloramphenicol acetyl transferase activity in transfected cells: a rapid and sensitive HPLC-based method

S L Young et al. DNA. 1985 Dec.

Abstract

An assay based on high-performance liquid chromatography (HPLC) has been developed for determining the expression of the acetyl coenzyme A: chloramphenicol 3-O-acetyltransferase gene in transfected cells. The assay is based on the separation of extracted, nonradioactive chloramphenicol and its acetylated derivatives by reverse-phase HPLC, using UV absorbance for detection. In addition to eliminating the need for costly [14C]chloramphenicol, this assay is as sensitive and much faster than the commonly used method involving thin-layer chromatography.

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