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. 2023 Sep 30;36(3):234-242.
eCollection 2023 Sep.

The Combination of Stromal Vascular Fraction Cells and Platelet-Rich Plasma Increases Fibroblast Growth Factor 7 and Promotes Angiogenesis in Full Thickness Burn Injury: Rat Experimental Study

F Josh  1 , T H Soekamto  1   2 , C A Windura  1
Affiliations

The Combination of Stromal Vascular Fraction Cells and Platelet-Rich Plasma Increases Fibroblast Growth Factor 7 and Promotes Angiogenesis in Full Thickness Burn Injury: Rat Experimental Study

F Josh et al. Ann Burns Fire Disasters. .

Abstract
in English, French

Our previous study on how the combination of stromal vascular fraction cells (SVFs) and platelet-rich plasma (PRP) affect deep dermal burn healing showed promising results. In this study, we assessed the effect on full-thickness burns by evaluating FGF7 serum level and capillary count. Forty-eight Wistar rats were divided into four major groups: (1) locally injected with combined SVFs and PRP; (2) topically applied Vaseline; (3) locally injected with placebo; (4) and rats without burns. These groups were divided further into smaller groups based on the day of euthanasia (8th hour, 4th day, 7th day, 14th day, and 21st day). FGF7 serum level was measured using ELISA, and capillaries were counted using a microscope. A one-way ANOVA test, post hoc, and regression tests were used. On day 4, both FGF7 and capillary counts showed significant differences between groups (p=0.000 and 0.048, respectively). On day 7, only FGF7 result showed a significant difference (p=0.000). On day 14, FGF7 and capillary counts showed significant differences (p=0.000, 0.018 respectively). The SVFs and PRP-treated groups showed superior results compared to other groups. The injection of combined SVFs and PRP increased FGF7 and capillary counts.

Notre précédente étude sur les effets de la combinaison de cFVS et de PRP sur la cicatrisation des brûlures, nous avons étudié les effets de cette même combinaison sur les brûlures profondes, en évaluant les niveaux de F7CF et le nombre de capillaires (NbC). Quarante-huit rats Wistar ont été répartis en 4 groupes : G1 = injection locale de cFVS et PRP ; G2 = vaseline topique ; G3 = injection locale de placebo ; G4 = rats sains. Ces groupes ont ensuite été subdivisés selon la date de sacrifice (8ème heure, 4ème, 7ème, 14ème et 21ème jours). F7CF a été mesuré par ELISA et les capillaires ont été comptés sous microscopie. Les analyses ont été effectuées par ANOVA unilatérale post hoc suivie de tests de régression. À J4, on constate une différence significative entre les groupes, tant pour F7CF (p= 0,000) que pour NbC (p= 0,048). À J7, la différence n’est significative que pour F7CF (p= 0,000). À J14, on retrouve une différence significative pour les 2 variables à l’étude (p= 0,000 et p= 0,018). Le groupe cFVS+PRP a des résultats meilleurs que les autres, cette injection augmentant F7CF et NbC.

Keywords: fibroblast growth factor 7; full thickness burn injury; platelet-rich plasma; stromal vascular fraction cells; wound healing.

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Figures

Fig. 1
Fig. 1
Histopathological results with HE staining, assessing the number of capillaries. A) 14th day treatment group; the arrow identifies a capillary; B) Positive control group on day 14; the arrow identifies a capillary; C) Negative control group on day 14; the arrow identifies a capillary; D) Normal control group; the arrow identifies a capillary
Fig. 2
Fig. 2
Histopathological result with HE staining showed full thickness skin destruction
Fig. 3
Fig. 3
Multiple comparison of FGF7
Fig. 4
Fig. 4
Multiple comparison of capillary count
See this image and copyright information in PMC

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