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Review
. 2024 Apr 12:15:1343255.
doi: 10.3389/fendo.2024.1343255. eCollection 2024.

Advancements in culture technology of adipose-derived stromal/stem cells: implications for diabetes and its complications

Yinze Shi #  1   2 , Xueyang Yang #  1   2 , Jie Min  1   2 , Wen Kong  1   2 , Xiang Hu  1   2 , Jiaoyue Zhang  1   2 , Lulu Chen  1   2
Affiliations
Review

Advancements in culture technology of adipose-derived stromal/stem cells: implications for diabetes and its complications

Yinze Shi et al. Front Endocrinol (Lausanne). .

Abstract

Stem cell-based therapies exhibit considerable promise in the treatment of diabetes and its complications. Extensive research has been dedicated to elucidate the characteristics and potential applications of adipose-derived stromal/stem cells (ASCs). Three-dimensional (3D) culture, characterized by rapid advancements, holds promise for efficacious treatment of diabetes and its complications. Notably, 3D cultured ASCs manifest enhanced cellular properties and functions compared to traditional monolayer-culture. In this review, the factors influencing the biological functions of ASCs during culture are summarized. Additionally, the effects of 3D cultured techniques on cellular properties compared to two-dimensional culture is described. Furthermore, the therapeutic potential of 3D cultured ASCs in diabetes and its complications are discussed to provide insights for future research.

Keywords: adipose-derived stem/stromal cells; diabetes mellitus; diabetic foot ulcer; monolayer culture; three-dimensional culture.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

Figure 1
Figure 1
The isolation process of ASCs. The cells showed are isolated from rat’s inguinal adipose tissue. Scale bar, 200μm. SVF, stromal vascular fraction; ASCs, adipose-derived stromal/stem cells.
Figure 2
Figure 2
Influential factors on biological functions of ASCs during culture. Many aspects are reported to influence ASC culture and their biological functions. These can broadly be divided into the sources of tissues and cells, techniques of isolation, culture and cryopreservation. WAT, white adipose tissue; BAT, brown adipose tissue; FBS, fetal bovine serum.
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