Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024:2744:503-514.
doi: 10.1007/978-1-0716-3581-0_30.

Recent Applications of FastFish-ID

Lawrence J Wangh  1   2 , John E Rice  3 , J Aquiles Sanchez  3
Affiliations

Recent Applications of FastFish-ID

Lawrence J Wangh et al. Methods Mol Biol. 2024.

Abstract

FastFish-ID via Closed-Tube barcoding is a portable platform for rapid and accurate identification of fish species that was conceived at Brandeis University, commercialized at Thermagenix, Inc., and further improved at Ecologenix, LLC (see Chap. 17 in this volume). This chapter focuses on the use of FastFish-ID for (1) identification of intraspecies variants, (2) quantitative use of FastFish-ID to measure the decay of fresh fish, and (3) use of FastFish-ID for the identification of dried and processed shark fins.

Keywords: Closed-Tube barcoding; DNA test for freshness; FastFish-ID; Fluorescent signature; Interdiction of Shark-Fin Trade; Intraspecies variants; Portable barcoding platform; Species identification; Synthetic Reference Target DNA.

PubMed Disclaimer

References

    1. Zhao L (2019) Mitochondrial DNA degradation: a quality control measure for mitochondrial genome maintenance and stress response. Enzyme 45:311–341. https://doi.org/10.1016/bs.enz.2019.08.004 - DOI
    1. Moretton A, Morel F, Macao B et al (2017) Selective mitochondrial DNA degradation following double-strand breaks. PLoS One 12(4):e0176795. https://doi.org/10.1371/journal.pone.0176795 - DOI - PubMed - PMC
    1. Anderson RR (2005) DNA degradation and postmortem interval: preliminary observations and methods. Master’s Thesis, University of Tennessee, https://trace.tennessee.edu/utk_gradthes/1582
    1. Desrochers E (2022) Conclusion of CITES leads to new protection of sharks and guitarfishes. SeafoodSource, December 1
    1. Prasetyo AP, Cusa M, Murray JM et al (2023) Universal closed-tube barcoding for 1 monitoring the shark and ray trade in megadiverse conservation hotspots. Doi: bioRxiv preprint https://doi.org/10.1101/2022.11.30.518468

LinkOut - more resources