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. 2024 May-Jun;38(3):1384-1389.
doi: 10.21873/invivo.13579.

Determinant Factors for Ongoing Pregnancy in Homologous Intrauterine Insemination Cycles - A Prospective Study

George Binas  1   2 , Vasilis Athanasiou  3 , Adamantios Athanasiou  3 , Ilianna Isihou  3 , Lampros-Orion Asimakopoulos  2 , Byron Asimakopoulos  2
Affiliations

Determinant Factors for Ongoing Pregnancy in Homologous Intrauterine Insemination Cycles - A Prospective Study

George Binas et al. In Vivo. 2024 May-Jun.

Abstract

Background/aim: Intrauterine insemination (IUI) is the most common assisted-reproduction treatment. However, it has lower success rate in comparison to other treatments. Therefore, determining factors that contribute to IUI success is of particular interest and this was the purpose of this prospective study.

Patients and methods: In this study, only homologous inseminations with fresh semen samples were included. All women received mild ovarian stimulation with clomiphene citrate and gonadotropins. Before IUI, basic semen analysis, evaluation of DNA fragmentation index (DFI), as well as measurement of sperm redox potential, were performed on each semen sample. Semen was processed with density-gradient centrifugation and 500 μl of processed sperm was used for insemination.

Results: In 200 cycles, there were 36 pregnancies, six of them ectopic. Cycles with ongoing pregnancies were characterized by younger male and female age and higher number of follicles. Multivariate logistic regression analysis showed that only female age was significantly associated with ongoing pregnancy. DFI was positively correlated with male age and negatively correlated with sperm concentration and progressive motility. Semen redox potential showed a strong negative correlation with sperm concentration and positive correlation with DFI.

Conclusion: Female age seems to be the most important determinant factor for the achievement of an ongoing pregnancy in homologous IUI cycles with fresh semen.

Keywords: DNA fragmentation; Intrauterine insemination; age; ongoing pregnancy; redox potential; semen.

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Conflict of interest statement

The Authors declare no potential conflicts of interest in relation to this study.

Figures

Figure 1
Figure 1. Male factor fertility status (N=200). A: Asthenozoospermia; AT: asthenoteratozoospermia; Normal: normozoospermia; O: oligozoospermia; OA: oligoasthenozoospermia; OAT: oligoasthenoteratozoospermia; OT: oligoteratozoospermia; T: teratozoospermia.
Figure 2
Figure 2. Female fertility status (N=200). PCOS: Polycystic ovarian syndrome.
Figure 3
Figure 3. Infertility status of the couples participating in the study (N=200).
See this image and copyright information in PMC

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References

    1. Chambers GM, Dyer S, Zegers-Hochschild F, De Mouzon J, Ishihara O, Banker M, Mansour R, Kupka MS, Adamson GD. International Committee for Monitoring Assisted Reproductive Technologies world report: assisted reproductive technology, 2014. Hum Reprod. 2021;36(11):2921–2934. doi: 10.1093/humrep/deab198. - DOI - PubMed
    1. European IVF Monitoring Consortium (EIM) for the European Society of Human Reproduction and Embryology (ESHRE), Smeenk J, Wyns C, De Geyter C, Kupka M, Bergh C, Cuevas Saiz I, De Neubourg D, Rezabek K, Tandler-Schneider A, Rugescu I, Goossens V. ART in Europe, 2019: results generated from European registries by ESHRE†. Hum Reprod. 2023;38(12):2321–2338. doi: 10.1093/humrep/dead197. - DOI - PMC - PubMed
    1. World Health Organization WHO Laboratory Manual for the Examination and Processing of Human Semen, Sixth Edition. Geneva, Switzerland, WHO. 2021 - PMC - PubMed
    1. Sun Y, Li B, Fan LQ, Zhu WB, Chen XJ, Feng JH, Yang CL, Zhang YH. Does sperm morphology affect the outcome of intrauterine insemination in patients with normal sperm concentration and motility. Andrologia. 2012;44(5):299–304. doi: 10.1111/j.1439-0272.2012.01280.x. - DOI - PubMed
    1. Demir B, Dilbaz B, Cinar O, Karadag B, Tasci Y, Kocak M, Dilbaz S, Goktolga U. Factors affecting pregnancy outcome of intrauterine insemination cycles in couples with favourable female characteristics. J Obstet Gynaecol. 2011;31(5):420–423. doi: 10.3109/01443615.2011.569780. - DOI - PubMed

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