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. 2024 Apr 17:12:1381738.
doi: 10.3389/fchem.2024.1381738. eCollection 2024.

Development of rapid nucleic acid testing techniques for common respiratory infectious diseases in the Chinese population

Shenshen Zhi  1 , Wenyan Wu  2 , Yan Ding  2 , Yuanyuan Zhang  2 , Liyan Pan  2 , Guo Liu  3 , Wei Li  2
Affiliations

Development of rapid nucleic acid testing techniques for common respiratory infectious diseases in the Chinese population

Shenshen Zhi et al. Front Chem. .

Abstract

Background: Most respiratory viruses can cause serious lower respiratory diseases at any age. Therefore, timely and accurate identification of respiratory viruses has become even more important. This study focused on the development of rapid nucleic acid testing techniques for common respiratory infectious diseases in the Chinese population. Methods: Multiplex fluorescent quantitative polymerase chain reaction (PCR) assays were developed and validated for the detection of respiratory pathogens including the novel coronavirus (SARS-CoV-2), influenza A virus (FluA), parainfluenza virus (PIV), and respiratory syncytial virus (RSV). Results: The assays demonstrated high specificity and sensitivity, allowing for the simultaneous detection of multiple pathogens in a single reaction. These techniques offer a rapid and reliable method for screening, diagnosis, and monitoring of respiratory pathogens. Conclusion: The implementation of these techniques might contribute to effective control and prevention measures, leading to improved patient care and public health outcomes in China. Further research and validation are needed to optimize and expand the application of these techniques to a wider range of respiratory pathogens and to enhance their utility in clinical and public health settings.

Keywords: fluorescent quantitative PCR; nucleic acid; public health; rapid testing; respiratory infections.

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Conflict of interest statement

Author GL was employed by Zeal Dental. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

FIGURE 1
FIGURE 1
Experimental scheme.
FIGURE 2
FIGURE 2
Baseline and threshold values for all primers.
FIGURE 3
FIGURE 3
Multiplex fluorescent quantitative PCR detection profiles for Group A—Novel Coronavirus (SARS-CoV-2). (A) Cq values (cycle numbers at the threshold ct) of amplified samples; (B) Dissociation curves and melting temperatures of amplification curves. ***p < 0.001, n = 6.
FIGURE 4
FIGURE 4
Multiplex fluorescent quantitative PCR detection profiles for Group B—Influenza A virus (FluA). (A) Cq values (cycle numbers at the threshold ct) of amplified samples; (B) Dissociation curves and melting temperatures of amplification curves. A total of 12 wells were run, including 6 replicates of actin samples and 6 replicates of FluA samples. ***p < 0.001, n = 6.
FIGURE 5
FIGURE 5
Multiplex fluorescent quantitative PCR detection profiles for Parainfluenza virus (PIV). (A) Cq values (cycle numbers at the threshold ct) of amplified samples; (B) Dissociation curves and melting temperatures of amplification curves. A total of 12 wells were run, including 6 replicates of actin samples and 6 replicates of PIV samples. ***p < 0.001, n = 6.
FIGURE 6
FIGURE 6
Multiplex fluorescent quantitative PCR detection profiles for Respiratory Syncytial Virus (RSV). (A) Cq values (cycle numbers at the threshold ct) of amplified samples; (B) Dissociation curves and melting temperatures of amplification curves. A total of 12 wells were run, including 6 replicates of actin samples and 6 replicates of RSV samples. ***p < 0.001, n = 6.
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