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. 2024;25(8):651-665.
doi: 10.2174/0113892037299818240408053000.

Influences of Ipomoea batatas Anti-Cancer Peptide on Tomato Defense Genes

Hsin-Hung Lin  1 , Kuan-Hung Lin  2 , Yung-Lin Tsai  3 , Rong-Jane Chen  4 , Yen-Chang Lin  5 , Yu-Chi Chen  3
Affiliations

Influences of Ipomoea batatas Anti-Cancer Peptide on Tomato Defense Genes

Hsin-Hung Lin et al. Curr Protein Pept Sci. 2024.

Abstract

Aims: This study investigates the impact of IbACP (Ipomoea batatas anti-cancer peptide) on defense-related gene expression in tomato leaves, focusing on its role in plant defense mechanisms.

Background: Previously, IbACP was isolated from sweet potato leaves, and it was identified as a peptide capable of inducing an alkalinization response in tomato suspension culture media. Additionally, IbACP was found to regulate the proliferation of human pancreatic adenocarcinoma cells.

Objective: Elucidate IbACP's molecular influence on defense-related gene expression in tomato leaves using next-generation sequencing analysis.

Methods: To assess the impact of IbACP on defense-related gene expression, transcriptome data were analyzed, encompassing various functional categories such as photosynthesis, metabolic processes, and plant defense. Semi-quantitative reverse-transcription polymerase chain reaction analysis was employed to verify transcription levels of defense-related genes in tomato leaves treated with IbACP for durations ranging from 0 h (control) to 24 h.

Results: IbACP induced jasmonic acid-related genes (LoxD and AOS) at 2 h, with a significant up-regulation of salicylic acid-dependent gene NPR1 at 24 h. This suggested a temporal antagonistic effect between jasmonic acid and salicylic acid during the early hours of IbACP treatment. Downstream ethylene-responsive regulator genes (ACO1, ETR4, and ERF1) were consistently down-regulated by IbACP at all times. Additionally, IbACP significantly up-regulated the gene expressions of suberization-associated anionic peroxidases (TMP1 and TAP2) at all time points, indicating enhanced suberization of the plant cell wall to prevent pathogen invasion.

Conclusion: IbACP enhances the synthesis of defense hormones and up-regulates downstream defense genes, improving the plant's resistance to biotic stresses.

Keywords: Ipomoea batatas anti-cancer peptide; Solanum lycopersicum; plant defense; plant peptides; tomato leaves.; transcriptomic analysis.

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