Positive impacts of Nannochloropsis oculata supplementation on gene expression of immune and antioxidant markers and metabolic profile of Barki sheep in the transition period and lipogenic effects on progeny

Abstract

Nannochloropsis species should be given priority when it comes to microalgae that should be added to feed since they are suitable for intense culture and have a high concentration of PUFAs (especially EPA), antioxidants, and certain vitamins. This study investigated the possible immune and antioxidant impacts of Nannochloropsis supplementation on Barki ewes during transition period and their newly born lambs. Three weeks prior to the expected time of lambing, the researched ewes were divided into two equal groups of thirty ewes each. The second group, on the other hand, was fed the same base diet as the first group plus 10 g of commercially available Nannochloropsis powder per kg of concentrate, given daily to each ewe's concentrate. Findings revealed that supplementation of ewes with Nannochloropsis significantly up-regulated the expression pattern of immune (NFKB, RANTES, HMGB1, TNF-α, IRF4, TLR7, CLA-DRB3.2, IL1B, IL6, CXCL8, S-LZ, and Cathelicidin), and antioxidant (SOD1, CAT, GPX1, GST, ATOX1, Nrf2 and AhpC/TSA) markers in ewes post-lambing and their newly born lambs. Additionally, mRNA levels of lipogenic (ACACA, FASN SCD, LPL, and BTN1A) markers were significantly up-regulated in lambs from supplemented ewes than control ones. There was a significant increase in the WBCs, Hb, RBc count, serum level of glucose, total protein, triacylglycerol and total cholesterol, GPx, catalase, IL1α and IL6 with significantly decreased serum level of TNF-α and MDA in supplemented ewes after lambing as compared with control ones. There was also a significant increase in WBCs, Hb, RBc count, birth weight and body temperature with significantly decreased in the serum levels of TNF-α and stillbirth of newly born lambs from supplemented ewes as compared to other lambs from control ones.

Keywords: Nannochloropsis oculata; Antioxidants; Immunity; Peripartum.

Fig. 1

Relative expression patterns of immunity genes in Barki ewes supplemented with Nannochloropsis algae before and after lambing. Results are expressed as means ± SEM. Small alphabetical letters show significance when (P < 0.05)

Fig. 2

Relative expression patterns of antioxidant genes in Barki ewes supplemented with Nannochloropsis algae before and after lambing. Results are expressed as means ± SEM. Small alphabetical letters show significance when (P < 0.05)

Fig. 3

Relative expression patterns of immunity genes in the newly born lambs from Barki ewes supplemented with Nannochloropsis algae. Results are expressed as means ± SEM. *P < 0.05

Fig. 4

Relative expression patterns of antioxidant genes in the newly born lambs from Barki ewes supplemented with Nannochloropsis algae. Results are expressed as means ± SEM. *P < 0.05

Fig. 5

Relative expression patterns of lipogenic genes in the newly born lambs from Barki ewes supplemented with Nannochloropsis algae. Results are expressed as means ± SEM. *P < 0.05