Effects of electroacupuncture on the neuronal activity of the arcuate nucleus of the rat hypothalamus

Abstract

Electroacupuncture (EA) effects on the spontaneous unit activities of 143 neurons in the hypothalamic arcuate nucleus (ARH) which were electrophysiologically identified to project to the median eminence were investigated using anesthetized rats weighing about 300 g. Stimuli were delivered unilaterally to a meridian point of Ho-Ku in the forepaw as rectangular pulses of 5 ms duration, intensity 300 to 500 microA, for 15 min at 3 and 45 Hz. Unit activities of ARH cells were extracellularly recorded and the mean firing rates were compared before and after EA stimulation. Stimulation at 3 or 45 Hz induced a long-lasting and naloxone-reversible suppression of the magnitude of the digastric electromyogram (dEMG) in the jaw opening reflex to 48 to 56% of the control value. Based upon the EA effects on the spontaneous firing rate, the ARH cells were classified into three types: the rate either increased (type I), decreased (type II), or did not change (type III). Type I, II, and III neurons composed 56, 40, and 4% of the recorded neurons (N = 45) when EA stimulation was applied at 3 Hz, and 27, 70, and 3% (N = 37) at 45 Hz, respectively. The distribution of the three types of ARH neurons after EA stimulation at 3 Hz was significantly different (P less than 0.05, chi-square test) from that at 45 Hz.