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. 2024 Feb 19:39:e00363.
doi: 10.1016/j.plabm.2024.e00363. eCollection 2024 Mar.

Serum reference intervals of micronutrients, vitamins, and interleukins among healthy adults in South-Western Nigeria

Tewogbade Adeoye Adedeji  1 , Nife Olamide Adedeji  2 , Olusola Akanni Jeje  3 , Abiodun Kofoworola Ajeigbe  1 , Olufemi Samuel Smith  3 , Temilola O Owojuyigbe  4 , Michael Bimbo Fawale  5 , Olabamiji Abiodun Ajose  1 , Simeon Adelani Adebisi  6 , Adeyinka Abdulrasak Akande  7 , Bashiru Adekunle Okesina  7
Affiliations

Serum reference intervals of micronutrients, vitamins, and interleukins among healthy adults in South-Western Nigeria

Tewogbade Adeoye Adedeji et al. Pract Lab Med. .

Abstract

Objectives: Clinical decision making depends mostly on appropriate application of numerical pathology reports from laboratory results, interpreted by comparison with reference intervals. We determined serum reference intervals of micronutrients, vitamins, and detectable interleukins among healthy adults in South-Western Nigeria.

Design and methods: This prospective study used a priori selection approach in blood-donors. They were screened for conditions that could elicit cytokine production.Serum micronutrients were assayed using Atomic Absorption Spectrophotometry; interleukins and vitamins by high Performance Liquid Chromatography. The reference intervals (RIs) were estimated at 2.5th percentile and 97.5th percentile.

Results: One hundred and eighteen (118) apparently healthy subjects, aged 18-56 years; 113 (95.8%) being 18-44years, and 5 (4.2%): 45-56 years; mostly males, 13 (11.02%) females, all Africans of Yoruba ethnicity.Estimated reference limits were: Zinc: 9.49-20.54 μmol/L, Selenium: 0.50-1.11 μmol/L, Copper: 13.86-27.97 μmol/L, Iron: 14.19-32.07 μmol/L, Manganese: 6.24-16.37 nmol/L; Magnesium: 0.78-1.62 mmol/L.Vitamins: A-1.08-2.39 μmol/L; D: 59.89-164.42 μmol/L; E: 7.13-19.45 μmol/L; K: 0.16-0.42 nmol/L; B1: 74.09-201.56 nmol/L; B6: 0.12-0.29 nmol/L; B12: 155.55-407.96 pmol/L; C: 47.74-112.99 μmol/L.Detected interleukins (IL-1 to IL-18): IL-1: 0.58-1.24 ng/L, IL-2: 0.09-0.18 ng/L, IL-3: 0.39-0.89 ng/L, IL-4: 0.27-0.58 ng/L, ….to IL-18: 0.74-1.56 ng/L.

Conclusions: The RI derived from this study for serum micronutrient, vitamin and interleukin concentrations are the first published for our population. They are in general agreement with those published from other geographical climes but there are differences at the lower and upper limits of the RI. The study reinforces the importance of deriving RI for the population that a clinical laboratory will serve.

Keywords: Interleukins; Micronutrients; Priori selection; Reference intervals; Vitamins.

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Conflict of interest statement

All authors declare that there are no conflicts of interest.

Figures

Figure 1
Figure 1
A sketch of overall methodology integration procedure
See this image and copyright information in PMC

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