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. 1985;19(1):57-61.
doi: 10.1007/BF00199313.

Tumour inhibitory effects of TCGF/IL-2/-containing preparations

J Bubeník, M Indrová, P Perlmann, K Berzins, O Mach, J Kraml, A Toulcová

Tumour inhibitory effects of TCGF/IL-2/-containing preparations

J Bubeník et al. Cancer Immunol Immunother. 1985.

Abstract

Supernatants from ConA-stimulated rat spleen cell cultures and from cultures of PMA-stimulated murine lymphoma subline EL-4TF were found to contain TCGF and to inhibit growth of a transplantable, MC-induced sarcoma MC11 in syngeneic mice. Tumour-inhibitory effects of the supernatants were dependent on local and repeated administration. Prior to use of the supernatants obtained from PMA-stimulated EL-4TF cell cultures, the dialysable PMA had to be removed; contamination with PMA was found to abolish the tumour-inhibitory effect of the supernatants and to produce enhancement of tumour growth. A significant tumour-inhibitory effect has also been obtained with partially purified TCGF prepared from culture supernatants of cloned EL-4TF cells by ammonium sulphate precipitation, ion-exchange (FPLC) chromatography, and AcA 44 Ultrogel filtration.

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