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. 2024 Oct;92(4):1658-1669.
doi: 10.1002/mrm.30144. Epub 2024 May 9.

Collagen degradation assessment with an in vitro rotator cuff tendinopathy model using multiparametric ultrashort-TE magnetization transfer (UTE-MT) imaging

Tan Guo  1   2 , Yan Song  2 , Jinlian Tong  3 , Sheng Jiao  2 , Cheng Shen  2 , Hong Wang  2 , Ju Cui  4 , Dapeng Dai  4 , Jie Ma  3 , Min Chen  1   2
Affiliations

Collagen degradation assessment with an in vitro rotator cuff tendinopathy model using multiparametric ultrashort-TE magnetization transfer (UTE-MT) imaging

Tan Guo et al. Magn Reson Med. 2024 Oct.

Abstract

Purpose: This study aims to assess ultrashort-TE magnetization transfer (UTE-MT) imaging of collagen degradation using an in vitro model of rotator cuff tendinopathy.

Methods: Thirty-six supraspinatus tendon specimens were divided into three groups and treated with 600 U collagenase (Group 1), 150 U collagenase (Group 2), and phosphate buffer saline (Group 3). UTE-MT imaging was performed to assess changes in macromolecular fraction (MMF), macromolecule transverse relaxation time (T2m), water longitudinal relaxation rate constant (R1m), the magnetization exchange rate from the macromolecular to water pool (Rm0 w) and from water to the macromolecular pool (Rm0 m), and magnetization transfer ratio (MTR) at baseline and following digestion and their differences between groups. Biochemical and histological studies were conducted to determine the extent of collagen degradation. Correlation analyses were performed with MMF, T2m, R1m, Rm0 w, Rm0 m, and MTR, respectively. Univariate and multivariate linear regression analyses were performed to evaluate combinations of UTE-MT parameters to predict collagen degradation.

Results: MMF, T2m, R1m, Rm0 m, and MTR decreased after digestion. MMF (r = -0.842, p < 0.001), MTR (r = -0.78, p < 0.001), and Rm0 m (r = -0.662, p < 0.001) were strongly negatively correlated with collagen degradation. The linear regression model of differences in MMF and Rm0 m before and after digestion explained 68.9% of collagen degradation variation in the tendon. The model of postdigestion in MMF and T2m and the model of MTR explained 54.2% and 52.3% of collagen degradation variation, respectively.

Conclusion: This study highlighted the potential of UTE-MT parameters for evaluation of supraspinatus tendinopathy.

Keywords: UTE; collagen degradation; magnetization transfer; rotator cuff tendinopathy.

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References

REFERENCES

    1. Allander E. Prevalence, incidence, and remission rates of some common rheumatic diseases or syndromes. Scand J Rheumatol. 1974;3:145‐153.
    1. Chard MD, Hazleman BL. Shoulder disorders in the elderly (a hospital study). Ann Rheum Dis. 1987;46:684‐687.
    1. Ashir A, Ma Y, Jerban S, et al. Rotator cuff tendon assessment in symptomatic and control groups using quantitative MRI. J Magn Reson Imaging. 2020;52:864‐872.
    1. Samiric T, Parkinson J, Ilic MZ, Cook J, Feller JA, Handley CJ. Changes in the composition of the extracellular matrix in patellar tendinopathy. Matrix Biol. 2009;28:230‐236.
    1. Riley GP, Harrall RL, Constant CR, Chard MD, Cawston TE, Hazleman BL. Tendon degeneration and chronic shoulder pain: changes in the collagen composition of the human rotator cuff tendons in rotator cuff tendinitis. Ann Rheum Dis. 1994;53:359‐366.

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