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. 2024:2799:47-54.
doi: 10.1007/978-1-0716-3830-9_4.

Transfection in Primary Cultured Neuronal Cells

Katie F M Marwick  1 , Giles E Hardingham  2
Affiliations

Transfection in Primary Cultured Neuronal Cells

Katie F M Marwick et al. Methods Mol Biol. 2024.

Abstract

Transfection allows the introduction of foreign nucleic acid into eukaryotic cells. It is an important tool in understanding the roles of NMDARs in neurons. Here we describe using lipofection-mediated transfection to introduce cDNA encoding NMDAR subunits into postmitotic rodent primary cortical neurons maintained in culture.

Keywords: Culture; Lipofection; NMDA; Neuron; Transfection.

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