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. 2024 Oct;87(10):2266-2274.
doi: 10.1002/jemt.24596. Epub 2024 May 11.

Smartphone microscopic method for imaging and quantification of microplastics in drinking water

Asmita Karki  1 , Bishan Man Thaiba  1 , K C Shishir Acharya  1 , Thakur Sedai  1 , Baburam Kandel  1 , Hari Paudyal  1 , Khaga Raj Sharma  1 , Basant Giri  2 , Bhanu Bhakta Neupane  1
Affiliations

Smartphone microscopic method for imaging and quantification of microplastics in drinking water

Asmita Karki et al. Microsc Res Tech. 2024 Oct.

Abstract

Analysis of microplastics in drinking water is often challenging due to smaller particle size and low particle count. In this study, we used a low cost and an easy to assemble smartphone microscopic system for imaging and quantitating microplastic particles as small as 20 μm. The system consisted of a spherical sapphire ball lens of 4 mm diameter attached to a smartphone camera as a major imaging component. It also involved pre-concentration of the sample using ZnCl2 solution. The spike recovery and limit of detection of the method in filtered distilled and deionized water samples (n = 9) were 55.6% ± 9.7% and 34 particles/L, respectively. Imaging performance of the microscopic system was similar to a commercial bright field microscopic system. The method was further implemented to examine microplastic particles in commercial bottled and jar water samples (n = 20). The particles count in bottled and jar water samples ranged from 0-91 particles/L to 0-130 particles/L, respectively. In both sample types, particles of diverse shape and size were observed. The particles collected from water samples were further confirmed by FTIR spectra (n = 36), which found 97% of the particles tested were made of plastic material. These findings suggested that the smartphone microscopic system can be implemented as a low-cost alternative for preliminary screening of microplastic in drinking water samples. RESEARCH HIGHLIGHTS: Ball lens based smartphone microscopic method was used for microplastic analysis. Particles of diverse shape and size were found in bottle and jar water samples.

Keywords: limit of detection; microplastic analysis; microplastic contamination; optical microscopy; smartphone microscopy.

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References

REFERENCES

    1. Achhammer, B. G., Reiney, M. J., & Reinhart, F. W. (1951). Study of degradation of polystyrene, using infrared. Journal of Research of the National Bureau of Standards, 47, 116.
    1. Alimi, O. S., Farner Budarz, J., Hernandez, L. M., & Tufenkji, N. (2018). Microplastics and nanoplastics in aquatic environments: Aggregation, deposition, and enhanced contaminant transport. Environmental Science & Technology, 52, 1704–1724.
    1. Arthur, C., Baker, J., & Bamford, H. (2009). Proceedings of the International Research Workshop on the Occurrence, Effects and Fate of Microplastic Marine Debris. National Oceanic and Atmospheric Administration Technical Memorandum. NOS‐OR&R‐30.
    1. Aryal, G. M., Aryal, B., Kandel, K. P., & Neupane, B. B. (2021). Cellulose‐based micro‐fibrous materials imaged with a home‐built smartphone microscope. Microscopy Research and Technique, 84, 1794–1801.
    1. Aslam, M., Qadir, A., Hafeez, S., Aslam, H. M. U., & Ahmad, S. R. (2022). Spatiotemporal dynamics of microplastics burden in river Ravi, Pakistan. Journal of Environmental Chemical Engineering, 10, 107652.

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