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Review
. 2024 May 1;19(3):031201.
doi: 10.1116/6.0003594.

Sum-frequency vibrational spectroscopy, a tutorial: Applications for the study of lipid membrane structure and dynamics

Joshua M Taylor  1 , John C Conboy  1
Affiliations
Review

Sum-frequency vibrational spectroscopy, a tutorial: Applications for the study of lipid membrane structure and dynamics

Joshua M Taylor et al. Biointerphases. .

Abstract

Planar supported lipid bilayers (PSLBs) are an ideal model for the study of lipid membrane structures and dynamics when using sum-frequency vibrational spectroscopy (SFVS). In this paper, we describe the construction of asymmetric PSLBs and the basic SFVS theory needed to understand and make measurements on these membranes. Several examples are presented, including the determination of phospholipid orientation and measuring phospholipid transmembrane translocation (flip-flop).

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Conflict of interest statement

The authors have no conflicts to disclose.

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