Coumarin-Modified Starch Fluorescent Nanoparticles as Sensor of Fe3+ and Zn2+ ions Utilizing Dynamic Quenching and Chelation Mechanisms

Abstract

Zinc and iron are two essential trace minerals that play a pivotal role in maintaining optimal health and well-being in the human body. Despite being required in relatively small quantities, their significance can be understated as they participate in a wide array of critical physiological processes such as oxygen transport, DNA synthesis, controlling nutrient availability, etc. Understanding the distribution and behavior of these ions in natural water bodies is essential for assessing water quality, studying ecological processes, and managing environmental impacts. In this study, we have developed a dual fluorescence probe using starch which was functionalized with coumarin derivatives, for efficient detection of Fe³⁺ and Zn²⁺ ions. This structure led a self-assembled starch/coumarin (SC) fluorescent nanoparticles with strong fluorescence intensity under ultraviolet light (365 nm). The quenching effect of Fe³⁺ on the SC fluorescent probe enabled efficient specific detection of Fe³⁺. Furthermore, Zn²⁺ ions increased fluorescence intensity of coumarin compounds (λ_emission = 459). This phenomenon occurs when the coumarin compound forms a complex or interacts with the zinc ion, resulting in enhanced fluorescence emission. In summary, the developed fluorescent probe offered a promising approach for sensitive and specific detection of iron and zinc ions in aqueous solutions.

Keywords: Amphiphilic nanoparticles; Corn starch; Coumarin; Fluorescent probe.