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. 2024:2807:175-194.
doi: 10.1007/978-1-0716-3862-0_13.

Measurement of HIV Rev-Rev Response Element Functional Activity

Patrick E H Jackson  1   2 , Godfrey Dzhivhuho  3   4 , Jing Huang  3   4 , Marie-Louise Hammarskjold  3   4 , David Rekosh  3   4
Affiliations

Measurement of HIV Rev-Rev Response Element Functional Activity

Patrick E H Jackson et al. Methods Mol Biol. 2024.

Abstract

Retroviruses must overcome cellular restrictions to the nucleocytoplasmic export of viral mRNAs that retain introns in order to complete their replication cycle. HIV accomplishes this using a system comprised of a trans-acting viral protein, Rev, and a cis-acting RNA secondary structure in the viral genome, the Rev-Response Element (RRE). HIV primary isolates differ with respect to the sequence and functional activity of the Rev-RRE system. Here, we describe a high throughput assay system for analyzing Rev-RRE functional activity using packageable viral vectors.

Keywords: HIV; LentivirusLentiviruses; RNA traffickingRNA trafficking; Rev; Rev response elementRev response element.

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References

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