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. 2024:2808:121-127.
doi: 10.1007/978-1-0716-3870-5_9.

Dual RNA-seq Analysis of Patients' Cells and Viral Genome After Measles Infection

Vasiliki Pogka  1 , Andreas Mentis  2 , Timokratis Karamitros  3
Affiliations

Dual RNA-seq Analysis of Patients' Cells and Viral Genome After Measles Infection

Vasiliki Pogka et al. Methods Mol Biol. 2024.

Abstract

During the infection of a host cell by an infectious agent, a series of gene expression changes occurs as a consequence of host-pathogen interactions. Unraveling this complex interplay is the key for understanding of microbial virulence and host response pathways, thus providing the basis for new molecular insights into the mechanisms of pathogenesis and the corresponding immune response. Dual RNA sequencing (dual RNA-seq) has been developed to simultaneously determine pathogen and host transcriptomes enabling both differential and coexpression analyses between the two partners as well as genome characterization in the case of RNA viruses. Here, we provide a detailed laboratory protocol and bioinformatics analysis guidelines for dual RNA-seq experiments focusing on - but not restricted to - measles virus (MeV) as a pathogen of interest. The application of dual RNA-seq technologies in MeV-infected patients can potentially provide valuable information on the structure of the viral RNA genome and on cellular innate immune responses and drive the discovery of new targets for antiviral therapy.

Keywords: Dual RNA-seq; Host response; Measles virus; Poly(A) enrichment; RNA viral genome; Ribodepletion.

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