Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2024 May 15;6(2):zcae024.
doi: 10.1093/narcan/zcae024. eCollection 2024 Jun.

Next generation sequencing technologies to address aberrant mRNA translation in cancer

Ángel-Carlos Román  1 , Dixan A Benítez  1 , Alba Díaz-Pizarro  1 , Nuria Del Valle-Del Pino  1 , Marcos Olivera-Gómez  1 , Guadalupe Cumplido-Laso  1 , Jose M Carvajal-González  1 , Sonia Mulero-Navarro  1
Affiliations
Review

Next generation sequencing technologies to address aberrant mRNA translation in cancer

Ángel-Carlos Román et al. NAR Cancer. .

Abstract

In this review, we explore the transformative impact of next generation sequencing technologies in the realm of translatomics (the study of how translational machinery acts on a genome-wide scale). Despite the expectation of a direct correlation between mRNA and protein content, the complex regulatory mechanisms that affect this relationship remark the limitations of standard RNA-seq approaches. Then, the review characterizes crucial techniques such as polysome profiling, ribo-seq, trap-seq, proximity-specific ribosome profiling, rnc-seq, tcp-seq, qti-seq and scRibo-seq. All these methods are summarized within the context of cancer research, shedding light on their applications in deciphering aberrant translation in cancer cells. In addition, we encompass databases and bioinformatic tools essential for researchers that want to address translatome analysis in the context of cancer biology.

PubMed Disclaimer

Figures

Graphical Abstract
Graphical Abstract
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Goodwin S., McPherson J.D., McCombie W.R. Coming of age: ten years of next-generation sequencing technologies. Nat. Rev. Genet. 2016; 17:333–351. - PMC - PubMed
    1. Shendure J., Ji H. Next-generation DNA sequencing. Nat. Biotechnol. 2008; 26:1135–1145. - PubMed
    1. Metzker M.L. Sequencing technologies — the next generation. Nat. Rev. Genet. 2009; 11:31–46. - PubMed
    1. Wang Z., Gerstein M., Snyder M. RNA-seq: a revolutionary tool for transcriptomics. Nat. Rev. Genet. 2009; 10:57–63. - PMC - PubMed
    1. Ozsolak F., Milos P.M. RNA sequencing: advances, challenges and opportunities. Nat. Rev. Genet. 2010; 12:87–98. - PMC - PubMed