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. 2023 Dec 31;33(8):1965-1974.
doi: 10.1007/s10068-023-01478-9. eCollection 2024 Jun.

Molecular characterization and antibiotic resistance of Staphylococcus aureus strains isolated from patients with diarrhea in Korea between the years 2007 and 2022

Young-Seok Bak  1 , Jun-Young Park  2 , Jung-Beom Kim  3 , Seung-Hak Cho  1   4   5
Affiliations

Molecular characterization and antibiotic resistance of Staphylococcus aureus strains isolated from patients with diarrhea in Korea between the years 2007 and 2022

Young-Seok Bak et al. Food Sci Biotechnol. .

Abstract

To investigate the molecular characteristics and antibiotic resistance of Staphylococcus aureus isolates from patients with diarrhea in Korea, 327 S. aureus strains were collected between 2007 and 2022. The presence of staphylococcal enterotoxin (SE) and toxic shock syndrome toxin-1 (TSST-1) genes in S. aureus isolates was determined by PCR. The highest expression of the TSST-1 gene was found in the GIMNO type (43.1% of GIMNO type). GIMNO type (Type I) refers to each staphylococcal enterotoxin (SE) gene gene (initials of genes): G = seg; I = sei; M = selm; N = seln; O = selo. Moreover, Type I isolates showed a significantly higher resistance to most antibiotics. A total of 195 GIMNO-type S. aureus strains were analyzed using multilocus sequence typing (MLST), and 18 unique sequence types (STs) were identified. The most frequent sequence type was ST72 (36.9%), followed by ST5 (22.1%) and ST30 (16.9%). Interestingly, ST72 strains showed a higher prevalence of MRSA than the other STs. In conclusion, our results were the first reported for S. aureus strains in Korea, which significantly expanded S. aureus genotype information for the surveillance of pathogenic S. aureus and may provide important epidemiological information to resolve several infectious diseases caused by S. aureus.

Supplementary information: The online version contains supplementary material available at 10.1007/s10068-023-01478-9.

Keywords: Antibiotic resistance; Diarrhea; Staphylococcus aureus.

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Conflict of interest statement

Conflict of interestThe authors declare no conflict of interest.

Figures

Fig. 1
Fig. 1
The occurrence of 17 toxin genes in 327 S.aureus clinical isolates
Fig. 2
Fig. 2
The resistance of 19 antibiotic agent following enterotoxin types. The percentage of isolates was determined by containing each antibiotic agent in isolates. (A). GIMNO type (Type I), (B) AHKQ type (Type II), (C) A type (Type III), (D) The enterotoxin genotypic and phenotypic trends year by year (2007–2022)
Fig. 3
Fig. 3
Percentage of sequence types (STs) in Type Ι group and major enterotoxin patterns according to clonal complex. (A) Percentage of sequence types (STs) in Type Ι group, (B) Major enterotoxin patterns according to clonal complex
Fig. 4
Fig. 4
MLST dendrogram of 195 egc-positive S. aureus isolates
See this image and copyright information in PMC

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