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. 2024 Aug;41(8):2053-2063.
doi: 10.1007/s10815-024-03132-7. Epub 2024 May 16.

The evaluation effect of nanoliposome-loaded Mito-Tempo on sperm parameters during human sperm cryopreservation

Rahil Jannatifar  1   2 , Hamid Piroozmanesh  3 , Seyedeh Saeideh Sahraei  4 , Atefeh Verdi  4 , Elham Asa  4
Affiliations

The evaluation effect of nanoliposome-loaded Mito-Tempo on sperm parameters during human sperm cryopreservation

Rahil Jannatifar et al. J Assist Reprod Genet. 2024 Aug.

Abstract

Aim: The aim of this study is the evaluation effect of nanoliposome-loaded Mito-Tempo on sperm parameters during human sperm cryopreservation.

Methods: Semen samples of 50 Asthenoteratozoospermia men (random) were collected. Sperm parameters were analyzed based on World Health Organization (WHO, 2010) criteria (2021) and each sample was divided into 5 groups (E1-E5). E1 (control group): the sperm was cryopreserved without nanoliposome, and Mito-Tempo. E2: sperm cryopreservation with Mito-Tempo-loaded nanoliposome (Mito-Tempo 0.1 mM) + freezing medium. E3: sperm cryopreservation with Mito-Tempo-loaded nanoliposome (Mito-Tempo 0.2 mM) + freezing medium. E4: in this group, the cryopreservation sperm with Mito-Tempo 0.3 mM + freezing medium. E5: the cryopreservation sperm with Mito-Tempo 0.2 mM + freezing medium.

Results: The result of this study indicated that sperm parameters and total antioxidant capacity (TAC) significantly increase in E3 and E4 groups, compared to E1, E2, and E5 groups respectively (P < 0.05). The percentage of abnormal morphology, DNA fragmentation index (DFI), malondialdehyde (MDA), and the levels of ROS significantly decrease in E3 and E4 groups, compared to E1, E2, and E5 groups (P < 0.05). In addition, the sperm parameters and stress oxidative factors significantly improve in E3 group compared to other groups (P < 0.05).

Conclusions: In conclusion, the combination of Mito-Tempo with nanoliposome due to its ability to cooperate with lipid layers may lead to significant performance in reducing oxidative stress damage and increasing the quality of sperm parameters.

Keywords: Cryopreservation; Mito-Tempo; Nanoliposome; Sperm.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
The flowchart of the study design
Fig. 2
Fig. 2
Effects of Mito-Tempo-loaded nanoliposomes on sperm motility. TMS, total motility sperm; PMS, progressive motility sperm; NMS, non-motility sperm; a: significantly with E1 (control group), b: significantly with other groups. P < 0.05; the values are expressed as mean ± SEM
Fig. 3
Fig. 3
Effects of Mito-Tempo-loaded nanoliposomes on sperm viability; a: significantly with E1 (control group); b: significantly with other groups P < 0.05 P < 0.05; the values are expressed as mean ± SEM
Fig. 4
Fig. 4
Effects of Mito-Tempo-loaded nanoliposomes on sperm DNA fragmentation; a: significantly with E1 (control group) b: significantly with other groups; The values are expressed as mean ± SEM
Fig. 5
Fig. 5
Sperm with red heads were considered to have intact DNA, and those with green heads were assumed to have fragmented DNA
Fig. 6
Fig. 6
Effects of Mito-Tempo-loaded nanoliposomes on sperm mitochondrial activity; a: significantly with E1 (control group); b: significantly with other groups P < 0.05; the values are expressed as mean ± SEM
Fig. 7
Fig. 7
Effects of Mito-Tempo-loaded nanoliposomes on sperm membrane integrity; a: significant difference VS. E1 (control group), b: significantly with other groups (p < 0 0.05). The values are expressed as mean ± SEM
Fig. 8
Fig. 8
Spermatozoa HOST negative (tail not coiled) (A). Spermatozoa classified as HOST positive (increasing coiling of the tail from) (B)
Fig. 9
Fig. 9
Effects of Mito-Tempo-loaded nanoliposomes on sperm oxidative stress status; a: significantly with E1 (control group); b: significantly with other groups (P < 0.05); the values are expressed as mean ± SEM
Fig. 10
Fig. 10
Effects of Mito-Tempo-loaded nanoliposomes on biochemical factors. MDA, malondialdehyde; TAC, total antioxidant capacity; a: significantly with E1 (control group); b: significantly with other groups (P < 0.05); the values are expressed as mean ± SEM
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