Antimicrobial susceptibility of Pneumocystis carinii in culture

Abstract

Pneumocystis carinii from infected rats were cultured on infected WI-38 human lung fibroblasts. After cultures were established, medium was replaced by medium containing antimicrobials and supernate organisms counted at specified times. Results show that metronidazole had no effect, amphotericin B suppressed organisms at 5 but not 0.5 microgram/ml, and miconazole, chloroquine, trimethoprim-sulfamethoxazole, and pentamidine isethionate suppressed organisms at the concentrations tested.