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. 2024:2775:171-193.
doi: 10.1007/978-1-0716-3722-7_12.

Interaction Between Macrophages and Cryptococcus neoformans: Distinguishing Phagocytosed Versus External Fungi

Amanda Pereira Rocha  1 , Thaís Silva Alves  1 , Adrielle Veloso Caixeta  1 , Patrícia Albuquerque  2 , André Moraes Nicola  3
Affiliations

Interaction Between Macrophages and Cryptococcus neoformans: Distinguishing Phagocytosed Versus External Fungi

Amanda Pereira Rocha et al. Methods Mol Biol. 2024.

Abstract

The interaction between macrophages and Cryptococcus neoformans is crucial in the pathogenesis of cryptococcosis. These phagocytes are important immune effectors, but also a niche in which facultative intracellular parasites, such as C. neoformans, thrive. Consequently, phagocytosis of cryptococcal cells and its outcomes are very frequently studied. One major issue with several of the tests used for this, however, is that macrophage-C. neoformans interaction does not always result in phagocytosis, as fungi may be attached to the external surface of the phagocyte. The most used methodologies to study phagocytosis of cryptococcal cells have varying degrees of precision in separating fungi that are truly internalized from those that are outside macrophages. Here we describe two assays to measure phagocytosis that can differentiate internal from external C. neoformans cells.

Keywords: Cryptococcosis; Cryptococcus neoformans; Flow cytometry; Fluorescence microscopy; Macrophage; Phagocytosis.

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