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. 2024:2775:359-365.
doi: 10.1007/978-1-0716-3722-7_23.

Analysis of Cryptococcus Extracellular Vesicles

Flavia C G Reis  1   2 , Rafael F Castelli  1 , Diogo Kuczera  1 , Haroldo C Oliveira  1 , Marcio L Rodrigues  3   4
Affiliations

Analysis of Cryptococcus Extracellular Vesicles

Flavia C G Reis et al. Methods Mol Biol. 2024.

Abstract

Extracellular vesicles (EVs) are produced by all domains of life. In fungal pathogens, they participate in virulence mechanisms and/or induce protective immunity, depending on the pathogenic species. EVs produced by pathogenic members of the Cryptococcus genus mediate virulence, antifungal resistance, as well as humoral and cell-mediated immunity. The isolation of cryptococcal EVs has been laborious and time-consuming for years. In this chapter, we detail a fast protocol for the isolation and analysis of EVs produced by members of the Cryptococcus genus.

Keywords: Cryptococcus; Electron microscopy; Extracellular vesicles; Nanoparticle tracking analysis; Solid media.

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References

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