[Non-protein sulfhydryl groups and glutathione-S-transferases in acute toxic effects of Di-n-alkyltin dichlorides in mice and rats]

Abstract

Toxic doses of di-n-butyl-tinn-dichloride (DBTC, 1.6 mmol/kg p.o., 0.04 mmol/kg i.v.) and of di-n-octyl-tinn-dichloride (DOTC, 2.4 mmol/kg p.o.) did not affect the non-protein sulfhydryl groups (NPSH) in mice livers. The NPSH depletion by a diethylmaleate pre-treatment did not influence acute toxic DOTC effects on the rats' thymus. DBTC concentrations of 10(-5) to 10(-4) mol/l in vitro inhibited the glutathion-S-transferase activity (GSH-Trs.) of cytosolic supernatants from the liver and the thymocytes of the rat compared with 1-chloro-2,4-dinitrobenzen as the electrophile substrate by max. 34%. The GSH-Trs. in the cytosolic liver supernatant had not been reduced after rats' DBTC treatment. A hydrophobic DBTC binding to GSH-Trs. is supposed.