Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Observational Study
. 2024 May 17;14(5):e075417.
doi: 10.1136/bmjopen-2023-075417.

Cohort profile of an early life observational cohort in China: Bone and MicroBiOme onset (BAMBOO) study

Jing Wang  1 , Chang Jiang  1 , Shuo Wang  1 , Lingyan Feng  1 , Yu Zhang  1 , Yuanyuan Guo  1 , Gongshu Liu  1 , Xi Li  2   3   4 , Guohong Zhang  3   4 , Xiaowei Zhu  3 , Fangyi Ren  3   5 , Lingyao Guan  3   5 , Jiayu Chen  3   5 , Ya Gao  3   4 , Mo Chen  6 , Noura Darwish  7 , Sara Colombo Mottaz  7 , Marie Noelle Horcajada  6 , Nicolas Bonnet  6 , Shaillay Kumar Dogra  6 , Dantong Wang  8
Affiliations
Observational Study

Cohort profile of an early life observational cohort in China: Bone and MicroBiOme onset (BAMBOO) study

Jing Wang et al. BMJ Open. .

Abstract

Purpose: The Bone And MicroBiOme Onset (BAMBOO) study is an ongoing prospective observational cohort study conducted in Tianjin, China, aiming to determine age-appropriate trajectories for microbiome maturation and bone development and to identify the influence of dietary factors in the process.

Participants: The recruitment started in September 2021 and was completed in February 2023. A total of 1380 subjects were recruited, 690 at birth (group 1) and 690 at 6 months of age (group 2). Groups 1 and 2 will be followed up for 12 months and 36 months, respectively.

Findings to date: The age of the mothers was 31.1±3.7 (mean±SD), and the birth weight of infants was 3.3±0.5 kg with an incidence of caesarean section 50.4%. Food diary information of the first 100 subjects showed that 64 food items were introduced by 6 months. A pilot microbiome analysis revealed that at the species level, bacterial communities were composed of mostly Bacteroides dorei, Bacteroides vulgatus and Escherichia coli, which were consistent with that of previous reports. Feasibility assessments of breast milk vitamin D and human milk oligosaccharides were validated through certified reference measurements. The early data assessment showed a high reliability of the data generated from this study.

Future plans: Data collection will be completed in August 2025. Four stage-statistical analyses will be performed as the cohort reaches certain age thresholds before the final report. Analysis of BAMBOO data will be used to develop age-appropriate trajectories for microbiome maturation and bone development for children aged 0-3 years and investigate the contribution of dietary factors in the process.

Trial registration number: ChiCTR2100049972.

Keywords: Microbiome; bone health; early life growth; early life nutrition.

PubMed Disclaimer

Conflict of interest statement

Competing interests: XL, GZ, XZ, FR, LG, JC and YG are employed by BGI Research. MC, ND, SCM, MNH, NB, SKD and DW are employed by Nestle Research. The authors declared that they have no competing interests.

Figures

Figure 1
Figure 1
Data and sample collection time points.
Figure 2
Figure 2
Biological sample collection and storage
Figure 3
Figure 3
Breakdown of drop-out reasons.
See this image and copyright information in PMC

Similar articles

See all similar articles

References

    1. Vijay A, Valdes AM. Role of the gut microbiome in chronic diseases: a narrative review. Eur J Clin Nutr 2022;76:489–501. 10.1038/s41430-021-00991-6 - DOI - PMC - PubMed
    1. Parekh PJ, Balart LA, Johnson DA. The influence of the gut microbiome on obesity, metabolic syndrome and gastrointestinal disease. Clin Transl Gastroenterol 2015;6:e91. 10.1038/ctg.2015.16 - DOI - PMC - PubMed
    1. Cong X, Xu W, Romisher R, et al. . Gut microbiome and infant health: brain-gut-microbiota axis and host genetic factors. Yale J Biol Med 2016;89:299–308. - PMC - PubMed
    1. Carlson AL, Xia K, Azcarate-Peril MA, et al. . Infant gut microbiome associated with cognitive development. Biol Psychiatry 2018;83:148–59. 10.1016/j.biopsych.2017.06.021 - DOI - PMC - PubMed
    1. Dogra SK, Kwong Chung C, Wang D, et al. . Nurturing the early life gut microbiome and immune maturation for long term health. Microorganisms 2021;9:2110. 10.3390/microorganisms9102110 - DOI - PMC - PubMed

Publication types

LinkOut - more resources