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. 1985 Jul;28(1):154-6.
doi: 10.1128/AAC.28.1.154.

A gene probe for TEM type beta-lactamases

R C Cooksey, N C Clark, C Thornsberry

A gene probe for TEM type beta-lactamases

R C Cooksey et al. Antimicrob Agents Chemother. 1985 Jul.

Abstract

A restriction fragment of plasmid pBR322 bearing the TEM-1 beta-lactamase structural gene was electroeluted from agarose gels after digestion with EcoRI and HinfI. The 1-kilobase fragment was 32P-labeled and used to examine genetic relationships with nucleic acids encoding seven other beta-lactamase classes. The probe hybridized only with TEM-2 and OXA-2 class plasmids.

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